Siglec-10 Protein, Human (Biotinylated, R119A, HEK293, His-Avi)

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (Biotinylated, R119A, HEK293, His-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Siglec-10 Protein, Human (Biotinylated, R119A, HEK293, His-Avi) is 530 a.a., with molecular weight of 68-80 kDa.

Siglec-10 protein, recognized as a putative adhesion molecule, functions in sialic-acid dependent cellular binding, displaying a preference for alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site of Siglec-10 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it appears to act as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation and recruiting cytoplasmic phosphatases via their SH2 domains, blocking signal transduction through dephosphorylation of signaling molecules. Siglec-10 is involved in the negative regulation of B-cell antigen receptor signaling, dependent on PTPN6/SHP-1. In association with CD24, it may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Siglec-10, in collaboration with CD24, regulates the immune response of natural killer (NK) cells and plays a role in the control of autoimmunity. During the initiation of adaptive immune responses by CD8-alpha(+) dendritic cells, Siglec-10 inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes, implicating the recruitment of PTPN6/SHP-1 and promoting phagosomal acidification. Siglec-10 interacts with various proteins, including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11.

1.Immobilized Anti-Siglec-10 Antibody hFc at 2 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) His with the EC₅₀ of 74.8 μg/mL determined by ELISA.
2.Immobilized Anti-Siglec-10 Antibody hFc at 5 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) His with the EC₅₀ of 61.7 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q96LC7-1 (M17-T546, R119A)

89790  [NCBI]

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST

68-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 200 mM L-Arginine, pH 8.0. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.