Siglec-8 Protein, Human (HEK293, His)

Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-6*His labeled tag.

Siglec-8 protein, identified as a putative adhesion molecule, serves as a mediator for sialic-acid dependent binding to red blood cells. It exhibits a preference for binding to alpha-2,3-linked sialic acid and also interacts with alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-8 may be concealed due to cis interactions with sialic acids present on the same cell surface. Furthermore, it has the ability to simultaneously recognize epitopes featuring a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a specific affinity for Gal-6-sulfated sialyl-Lewis X glycan epitopes.

Human

HEK293

C-6*His

Q9P0M4/NP_037410.1 (M17-A363)

27181  [NCBI]

MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA

40-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.