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  4. SLC44A1 Protein, Human (HEK293)

The SLC44A1 protein acts as a choline/H+ and high-affinity ethanolamine/H+ antiporter and is critical for regulating cellular choline and ethanolamine transport. Its dual function actively redistributes intracellular ethanolamine and balances the CDP-Cho and CDP-Etn arms of the Kennedy pathway. SLC44A1 Protein, Human (HEK293) is the recombinant human-derived SLC44A1 protein, expressed by HEK293.

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Size Price Stock Quantity
10 μg USD 910 Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μg USD 2550 Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  

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Description

The SLC44A1 protein acts as a choline/H+ and high-affinity ethanolamine/H+ antiporter and is critical for regulating cellular choline and ethanolamine transport. Its dual function actively redistributes intracellular ethanolamine and balances the CDP-Cho and CDP-Etn arms of the Kennedy pathway. SLC44A1 Protein, Human (HEK293) is the recombinant human-derived SLC44A1 protein, expressed by HEK293.
Background

SLC44A1 protein functions as a choline/H+ antiporter, playing a pivotal role in the regulation of cellular choline transport as documented in various studies. Additionally, it acts as a high-affinity ethanolamine/H+ antiporter, thereby controlling the supply of extracellular ethanolamine for the CDP-Etn pathway. This dual functionality allows SLC44A1 to actively redistribute intracellular ethanolamine, maintaining a balance between the CDP-Cho and CDP-Etn arms of the Kennedy pathway. The involvement of SLC44A1 in these processes is crucial for membrane synthesis and myelin production, highlighting its significance in fundamental cellular functions and suggesting potential implications for membrane homeostasis and neural development.
Species

Human
Source

HEK293
Tag

Tag Free
Accession

Q8WWI5 (M1-A657)
Gene ID

23446
Molecular Construction
N-term
SLC44A1 (M1-A657)
Accession # Q8WWI5-1
C-term
Protein Length

Full Length
Synonyms
SLC44A1; Choline transporter-like protein 1; CDw92; Solute carrier family 44 member 1; CD92
AA Sequence

MGCCSSASSAAQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLVSGYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGVMTSKEIILGLCLLSLVLSMILMVIIRYISRVLVWILTILVILGSLGGTGVLWWLYAKQRRSPKETVTPEQLQIAEDNLRALLIYAISATVFTVILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWVYWIMTLLFLGTTGSPVQNEQGFVEFKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDKRNLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCVLKSCICCLWCLEKCLNYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVATINTVGDFMLFLGKVLIVCSTGLAGIMLLNYQQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGREFYMDKVLMEFVENSRKAMKEAGKGGVADSRELKPMASGASSA

Predicted Molecular Mass
74 kDa
Molecular Weight

Approximately 56 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 80% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of 20 mM HEPES pH7.5, 300 mM NaCl, 0.03% (w/v) DDM, 0.003% (w/v) CHS
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

Please use rapid thawing with running water to thaw the protein.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice
Documentation
Data Sheet (239 KB) Handling Instructions (2659 KB)

SLC44A1 Protein, Human (HEK293) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that the storage concentration after dilution is not less than 100 μg/mL. 3. Aliquot at least 20 μL per tube. 4. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorporated into the protein storage solution.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SLC44A1 Protein, Human (HEK293)
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