2. Recombinant Proteins
  3. SMPD1 Protein, Mouse (sf9, C-His)

SMPD1 Protein, Mouse (sf9, C-His)

Cat. No.: HY-P76134A
Handling Instructions

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, C-His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, C-His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background

The SMPD1 protein functions by converting sphingomyelin into ceramide through two enzymatic forms derived from alternative trafficking of a single precursor protein. One form is targeted to the endolysosomal compartment, while the other is released extracellularly. However, during stress responses, lysosomal exocytosis may serve as a significant source of the secretory form. Within lysosomes, it carries out the conversion of sphingomyelin to ceramide and plays a crucial role in exporting cholesterol from intraendolysosomal membranes. Additionally, SMPD1 exhibits phospholipase C activities towards 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Its involvement in stress-induced apoptosis modulation is mediated through ceramide production.
Species

Mouse
Source

Sf9 insect cells
Tag

C-10*His
Accession

Q04519 (L45-L626)
Gene ID

20597
Molecular Construction
N-term
SMPD1 (M1-L626)
Accession # Q04519
His
C-term
Synonyms
Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASMase; Smpd1; Asm
AA Sequence

LFDSTVLWVPARAYPLPSEGHSVKFSAIAPPLQSAFGWQNLTCPACKVLFTALNHGLKKEPNVARVGSVAIKICKMLNIAPLDVCQSAVHLFEDDVVEVWTRSVLSPSEACGLLLGSSCGHWDIFSTWNISLPSVPKPPPKPPSPPAPGAPVSRVLFLTDLHWDHEYLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLPLRTLESLLKGLGPAGPFEMVYWTGDIPAHDVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFIKGNQSSQWLYEAMAKAWEPWLPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHIIGHIPPGHCLKSWSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDEETLSRPLAVAFLAPSATTFINLNPGYRVYQIDGNYPGSSHVVLDHETYILNLTQANAAGGTPSWKRLYRARETYGLPDAMPASWHNLVYRMRDDEQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPNGSLPDANRLWSRPLL

Molecular Weight

Approximately 68-71 kDa due to the glycosylation.
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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