SMPD1 Protein, Mouse (sf9, C-His)

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, C-His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

The SMPD1 protein functions by converting sphingomyelin into ceramide through two enzymatic forms derived from alternative trafficking of a single precursor protein. One form is targeted to the endolysosomal compartment, while the other is released extracellularly. However, during stress responses, lysosomal exocytosis may serve as a significant source of the secretory form. Within lysosomes, it carries out the conversion of sphingomyelin to ceramide and plays a crucial role in exporting cholesterol from intraendolysosomal membranes. Additionally, SMPD1 exhibits phospholipase C activities towards 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Its involvement in stress-induced apoptosis modulation is mediated through ceramide production.

Mouse

Sf9 insect cells

C-10*His

Q04519 (L45-L626)

20597

LFDSTVLWVPARAYPLPSEGHSVKFSAIAPPLQSAFGWQNLTCPACKVLFTALNHGLKKEPNVARVGSVAIKICKMLNIAPLDVCQSAVHLFEDDVVEVWTRSVLSPSEACGLLLGSSCGHWDIFSTWNISLPSVPKPPPKPPSPPAPGAPVSRVLFLTDLHWDHEYLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLPLRTLESLLKGLGPAGPFEMVYWTGDIPAHDVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFIKGNQSSQWLYEAMAKAWEPWLPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHIIGHIPPGHCLKSWSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDEETLSRPLAVAFLAPSATTFINLNPGYRVYQIDGNYPGSSHVVLDHETYILNLTQANAAGGTPSWKRLYRARETYGLPDAMPASWHNLVYRMRDDEQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPNGSLPDANRLWSRPLL

Approximately 68-71 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 0.1% Tween80, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.