SMPDL3A Protein, Human (HEK293, His)

SMPDL3A exhibits in vitro nucleotide phosphodiesterase activity, specifically toward ATP and p-nitrophenyl-TMP, less activity toward nucleoside diphosphates, and no activity toward nucleoside monophosphates. Notably, it showed in vitro activity with CDP-choline and CDP-ethanolamine, producing specific products. SMPDL3A Protein, Human (HEK293, His) is the recombinant human-derived SMPDL3A protein, expressed by HEK293 , with C-6*His labeled tag.

SMPDL3A exhibits in vitro nucleotide phosphodiesterase activity, particularly with nucleoside triphosphates such as ATP, as well as p-nitrophenyl-TMP. While it displays lower activity with nucleoside diphosphates, no activity is observed with nucleoside monophosphates. Notably, SMPDL3A demonstrates in vitro activity with CDP-choline, producing CMP and phosphocholine, as well as with CDP-ethanolamine. However, it does not possess sphingomyelin phosphodiesterase activity. These enzymatic characteristics highlight the substrate specificity and potential physiological relevance of SMPDL3A in nucleotide phosphodiester hydrolysis.

Human

HEK293

C-6*His

Q92484-1 (L23-Y453)

10924  [NCBI]

LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY

Approximately 59.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.