SPHK1/Sphingosine Kinase 1 Protein, Human (sf9, His-GST)

The SPHK1/sphingosine kinase 1 protein is a multifunctional lipid mediator that phosphorylates sphingosine to generate sphingosine-1-phosphate (SPP). SPHK1/Sphingosine Kinase 1 Protein, Human (sf9, His-GST) is the recombinant human-derived SPHK1/Sphingosine Kinase 1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SPHK1/Sphingosine Kinase 1 Protein, Human (sf9, His-GST) is 384 a.a., with molecular weight of ~64 kDa.

The SPHK1 (Sphingosine Kinase 1) protein serves as a critical mediator in cellular signaling by catalyzing the phosphorylation of sphingosine, generating sphingosine 1-phosphate (SPP), a versatile lipid mediator with both intra- and extracellular functions. While also acting on D-erythro-sphingosine and sphinganine to a lesser extent, SPHK1 exhibits specificity by not affecting other lipids such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to its proapoptotic counterpart SPHK2, SPHK1 negatively influences intracellular ceramide levels, promoting cell growth and inhibiting apoptosis. Its involvement extends to the regulation of inflammatory responses and neuroinflammation, where SPP stimulates TRAF2 E3 ubiquitin ligase activity, leading to NF-kappa-B activation and IL17 secretion in response to TNF signaling. Additionally, SPHK1 negatively regulates RANTES induction through the p38 MAPK signaling pathway. Beyond its kinase activity, SPHK1 plays a role in endocytic membrane trafficking, endosomal maturation, and membrane fusion. In Purkinje cells, it is implicated in the regulation of autophagosome-lysosome fusion upon VEGFA stimulation. Notably, SPHK1 exhibits serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner, promoting the neuronal secretion of specialized preresolving mediators, particularly 15-R-lipoxin A4, during neuroinflammation, which enhances phagocytic microglia.

Human

Sf9 insect cells

N-His;N-GST

Q9NYA1 (M1-L384)

8877  [NCBI]

Approximately 64 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.