ST2/IL1RL1 Protein, Cynomolgus (HEK293, His)

ST2/IL1RL1 Protein is a member of the Toll-like receptor superfamily, and is a soluble and membrane-bound protein that is the receptor for interleukin 33. It recruits MYD88, IRAK1/4, and TRAF6 after stimulation, leading to MAPK phosphorylation. It is also an inhibitor of interleukin 1 receptor (IL-1RI) and Toll-like receptor 4 (TLR4) signaling, maintaining endotoxin tolerance. ST2/IL1RL1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-His labeled tag.

ST2/IL1RL1 Protein is a member of the toll-like receptor superfamily, but unlike other members, it does not induce inflammatory responses by activating NF-κB, although it can activate MAP kinase. ST2/IL1RL1 Protein exists in two forms (soluble and membrane-bound forms). When the heart muscle is stretched, the ST2 gene is upregulated, and the circulating soluble ST2/IL1RL1 Protein concentration rapidly increases. Its membrane-bound form serves as a receptor for IL-33, which binds to IL-33 to regulate cardiac diseases and injuries, such as ischemic events, triggering a cardioprotective effect and maintaining cardiac function. The membrane-bound form of ST2/IL1RL1 Protein negatively regulates the signaling transduction of type I interleukin 1 receptor (IL-1RI) and toll-like receptor 4 (TLR4) by sequestering the adaptor MyD88 and Mal. It can maintain endotoxin tolerance^[1][2].
Overexpression of ST2/IL1RL1 Protein increases the production of neutrophil chemokine IL-8 and enhances HRV1B-induced IP-10 (a chemokine involved in exacerbation of asthma). ST2/IL1RL1 Protein also promotes proinflammatory responses to airway bacterial and viral infections. Therefore, ST2/IL1RL1 Protein plays an important role in inflammation regulation^[3].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Cynomolgus

HEK293

C-His

XP_005575214.1 (M1-C331)

102115135  [NCBI]

Approximately 37.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.