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  4. SULT2B1 Protein, Human (311a.a, C-His)

SULT2B1 Protein, Human (311a.a, C-His)

Cat. No.: HY-P71011A
COA Handling Instructions

The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA.It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation.SULT2B1 Protein, Human (C-His) is the recombinant human-derived SULT2B1 protein, expressed by E.coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA.It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation.SULT2B1 Protein, Human (C-His) is the recombinant human-derived SULT2B1 protein, expressed by E.coli , with C-6*His labeled tag.

Background

SULT2B1 protein, a sulfotransferase utilizing 3'-phospho-5'-adenylyl sulfate (PAPS) as its sulfonate donor, holds a pivotal role in catalyzing sulfate conjugation, with a primary responsibility for sulfating cholesterol. Additionally, SULT2B1 demonstrates significant activity in sulfating steroids, particularly the 3beta-hydroxyl groups of molecules such as pregnenolone and dehydroepiandrosterone (DHEA). While it exhibits a preferential sulfonation of cholesterol, it also displays substantial activity with pregnenolone and DHEA. Beyond its enzymatic functions, SULT2B1 plays a crucial role in epidermal cholesterol metabolism, contributing to the regulation of epidermal proliferation and differentiation. Notably, it sulfonates pregnenolone but not cholesterol, underscoring its substrate selectivity and emphasizing its multifaceted involvement in the intricate processes governing cholesterol homeostasis and steroid metabolism.

Biological Activity

Measured by its ability to transfer sulfate from PAPS to pregnenolone. The specific activity is 17.167 pmol/min/μg that incubate at 37 °C for 30 minutes.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

O00204-1 (M1-E311)

Gene ID

6820

Molecular Construction
N-term
SULT2B1 (M1-E311)
Accession # O00204-1
6*His
C-term
Synonyms
Sulfotransferase Family Cytosolic 2B Member 1; ST2B1; Sulfotransferase 2B1; Alcohol Sulfotransferase; Hydroxysteroid Sulfotransferase 2; SULT2B1; HSST2
AA Sequence

MDGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDE

Molecular Weight

Approximately 37.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

SULT2B1 Protein, Human (311a.a, C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SULT2B1 Protein, Human (311a.a, C-His)
Cat. No.:
HY-P71011A
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