TFRC Protein, Mouse (HEK293, His)

TFRC is a key protein in cellular iron uptake, coordinating receptor-mediated endocytosis of the ligand transferrin into specialized endosomes, where endosomal acidification triggers iron release.TFRC actively regulates T and B cell proliferation through iron uptake.TFRC Protein, Mouse (HEK293, His) is the recombinant mouse-derived TFRC protein, expressed by HEK293 , with N-8*His labeled tag.

TFRC, a pivotal protein in cellular iron uptake, orchestrates receptor-mediated endocytosis of ligand-occupied transferrin into specialized endosomes, where endosomal acidification triggers iron release. The apotransferrin-receptor complex undergoes recycling to the cell surface while a return to neutral pH, leading to the loss of apotransferrin affinity for its receptor. Essential for erythrocyte and nervous system development, TFRC positively regulates T and B cell proliferation through iron uptake. Acting as a lipid sensor, TFRC modulates mitochondrial fusion by regulating the JNK pathway. Low dietary stearate levels induce JNK pathway activation, resulting in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2, inhibiting mitochondrial fusion. Conversely, high stearate levels stearoylate TFRC, suppressing JNK pathway activation and preventing MFN2 degradation. TFRC also can function as a homodimer with disulfide linkage, binding one transferrin molecule per subunit, and interacts with SH3BP4 and STEAP3, the latter facilitating TFRC endocytosis in erythroid precursor cells.

Mouse

HEK293

N-8*His

Q62351 (C89-F763)

22042  [NCBI]

CKRVEQKEECVKLAETEETDKSETMETEDVPTSSRLYWADLKTLLSEKLNSIEFADTIKQLSQNTYTPREAGSQKDESLAYYIENQFHEFKFSKVWRDEHYVKIQVKSSIGQNMVTIVQSNGNLDPVESPEGYVAFSKPTEVSGKLVHANFGTKKDFEELSYSVNGSLVIVRAGEITFAEKVANAQSFNAIGVLIYMDKNKFPVVEADLALFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGKMEGSCPARWNIDSSCKLELSQNQNVKLIVKNVLKERRILNIFGVIKGYEEPDRYVVVGAQRDALGAGVAAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDFGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPVDGKSLYRDSNWISKVEKLSFDNAAYPFLAYSGIPAVSFCFCEDADYPYLGTRLDTYEALTQKVPQLNQMVRTAAEVAGQLIIKLTHDVELNLDYEMYNSKLLSFMKDLNQFKTDIRDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLKLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.