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  6. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His)

TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His)

Cat. No.: HY-P78668
COA Handling Instructions

TGF beta 1 is a secreted ligand of the TGF-beta superfamily that binds to receptors and activates SMAD transcription factors. Preproprotein is proteolytically processed to produce latency-associated peptide (LAP) and mature peptide. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His) is the recombinant human-derived TGF beta 1/TGFB1 Latency-associated peptide protein, expressed by HEK293 , with N-6*His labeled tag and C33S mutation.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

TGF beta 1 is a secreted ligand of the TGF-beta superfamily that binds to receptors and activates SMAD transcription factors. Preproprotein is proteolytically processed to produce latency-associated peptide (LAP) and mature peptide. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His) is the recombinant human-derived TGF beta 1/TGFB1 Latency-associated peptide protein, expressed by HEK293 , with N-6*His labeled tag and C33S mutation.

Background

TGF beta 1 Protein, the product of this gene, encodes a secreted ligand belonging to the TGF-beta superfamily, known for its role in regulating various cellular processes. As a ligand, it binds to different TGF-beta receptors, initiating the recruitment and activation of SMAD family transcription factors that govern gene expression. The encoded preproprotein undergoes proteolytic processing, yielding a latency-associated peptide (LAP) and a mature peptide. It exists in either a latent form, comprising a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. This protein, which can form heterodimers with other TGFB family members, plays a pivotal role in regulating cell proliferation, differentiation, and growth. Additionally, it has the capacity to modulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. Frequently upregulated in tumor cells, mutations in this gene lead to Camurati-Engelmann disease. Its expression is broadly observed in various tissues, with prominent levels in the spleen (RPKM 38.0), bone marrow (RPKM 32.1), and 23 other tissues, highlighting its widespread involvement in physiological processes.

Biological Activity

Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. The ED 50 for this effect is 55.73 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF beta 1,corresponding to a specific activity is 1.79×10^4 units/mg.

Species

Human

Source

HEK293

Tag

N-6*His

Accession

P01137/NP_000651.3 (L30-R278, C33S)

Gene ID
Molecular Construction
N-term
6*His
TGF beta 1 (L30-R278, C33S)
Accession # NP_000651.3
C-term
Synonyms
LAP (TGF-beta 1); LAP (TGFB1); TGFB1; CED; DPD1; LAP; TGF-beta-1; TGFB
AA Sequence

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR

Molecular Weight

37-44 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His)
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