TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His)

TGF beta 1 is a secreted ligand of the TGF-beta superfamily that binds to receptors and activates SMAD transcription factors. Preproprotein is proteolytically processed to produce latency-associated peptide (LAP) and mature peptide. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His) is the recombinant human-derived TGF beta 1/TGFB1 Latency-associated peptide protein, expressed by HEK293 , with N-6*His labeled tag and C33S mutation.

TGF beta 1 Protein, the product of this gene, encodes a secreted ligand belonging to the TGF-beta superfamily, known for its role in regulating various cellular processes. As a ligand, it binds to different TGF-beta receptors, initiating the recruitment and activation of SMAD family transcription factors that govern gene expression. The encoded preproprotein undergoes proteolytic processing, yielding a latency-associated peptide (LAP) and a mature peptide. It exists in either a latent form, comprising a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. This protein, which can form heterodimers with other TGFB family members, plays a pivotal role in regulating cell proliferation, differentiation, and growth. Additionally, it has the capacity to modulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. Frequently upregulated in tumor cells, mutations in this gene lead to Camurati-Engelmann disease. Its expression is broadly observed in various tissues, with prominent levels in the spleen (RPKM 38.0), bone marrow (RPKM 32.1), and 23 other tissues, highlighting its widespread involvement in physiological processes.

Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. The ED ₅₀ for this effect is 55.73 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF beta 1,corresponding to a specific activity is 1.79×10^4 units/mg.

Human

HEK293

N-6*His

P01137/NP_000651.3 (L30-R278, C33S)

7040  [NCBI]

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR

37-44 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.