THOP1 Protein, Human (His)

The THOP1 protein metabolizes short neuropeptides, degrading them in the cytoplasm. It also degrades amyloid precursor protein, producing fragments associated with amyloid production. THOP1 Protein, Human (His) is the recombinant human-derived THOP1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of THOP1 Protein, Human (His) is 688 a.a., with molecular weight of 80-85 kDa.

THOP1 Protein plays a crucial role in the metabolism of neuropeptides with a length of fewer than 20 amino acid residues, participating specifically in cytoplasmic peptide degradation. Its enzymatic activity extends to the degradation of the amyloid-beta precursor protein, generating fragments associated with amyloidogenesis. Additionally, THOP1 serves as a regulatory component in the cannabinoid signaling pathway, where it facilitates the degradation of hemopressin, an antagonist peptide targeting the cannabinoid receptor CNR1. The diverse functions of THOP1 underscore its involvement in intricate cellular processes, ranging from neuropeptide metabolism to the modulation of key signaling pathways.

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGPK(Dnp)-OH that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 2815.75 pmol/min/µg.

Human

E. coli

C-6*His

P52888 (K2-C689)

7064  [NCBI]

KPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQVGTQEFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPSKDIRTASTEADKKLSEFDVEMSMREDVYQRIVWLQEKVQKDSLRPEAARYLERLIKLGRRNGLHLPRETQENIKRIKKKLSLLCIDFNKNLNEDTTFLPFTLQELGGLPEDFLNSLEKMEDGKLKVTLKYPHYFPLLKKCHVPETRRKVEEAFNCRCKEENCAILKELVTLRAQKSRLLGFHTHADYVLEMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKRAECERRGLPFDGRIRAWDMRYYMNQVEETRYCVDQNLLKEYFPVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARDAASGEVVGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQIAIAAMVANFTKPTADAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLIESRQANTGLFNLRQIVLAKVDQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLNSKVGMDYRSCILRPGGSEDASAMLRRFLGRDPKQDAFLLSKGLQVGGCEPEPQVC

80-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 50% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.