TIGIT Protein, Human (HEK293, His)

The TIGIT protein plays a key role in immune regulation and binds with high affinity to the poliovirus receptor (PVR). This interaction induces an immunosuppressive environment by increasing IL10 secretion and decreasing IL12B secretion. TIGIT Protein, Human (HEK293, His) is the recombinant human-derived TIGIT protein, expressed by HEK293 , with C-6*His labeled tag.

The TIGIT protein plays a pivotal role in immune regulation, exhibiting high-affinity binding to the poliovirus receptor (PVR). This interaction leads to increased secretion of IL10 and decreased secretion of IL12B, contributing to an immunosuppressive environment. TIGIT further exerts its immunomodulatory effect by suppressing T-cell activation and promoting the generation of mature immunoregulatory dendritic cells. Structurally, TIGIT forms a homodimer in cis, binding with high affinity to PVR, thereby creating a heterotetrameric assembly comprising two TIGIT and two PVR molecules. Additionally, TIGIT demonstrates lower-affinity binding to NECTIN2 and NECTIN3, underscoring its capacity for diverse molecular interactions. The multifaceted functions and binding affinities of TIGIT highlight its crucial role in immune regulation and its potential as a therapeutic target in modulating immune responses.

Human

HEK293

C-6*His

Q495A1-1 (M22-P141)

201633  [NCBI]

MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP

16-18 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.