TIM-3/HAVCR2 Protein, Human (HEK293, Fc)

TIM3 Protein, Human (HEK293, Fc) is a type I transmembrane glycoprotein belonging to T Cell Immunoglobulin Mucin (TIM) family, and is involved in regulating innate and adaptive immunity.

T Cell Immunoglobulin Mucin 3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Human TIM-3 consists of a membrane distal variable immunoglobulin (IgV)-like domain involved in ligand interactions, a membrane proximal mucin domain and a transmembrane domain that is connected to an intracellular cytoplasmic tail involved in phosphotyrosine-dependent signaling^[1][2][3].

1.Immobilized TIM-3, hFc, Human at 0.5 μg/mL, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/mL.
2.Immobilized Galectin-9, His, Human at 0.5 μg/mL (100 µL/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/mL.

Human

HEK293

C-hFc

Q8TDQ0-1 (S22-R200)

84868  [NCBI]

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

60-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Homayouni V, et al. Stimulation of Camel Polyclonal Antibody against Human T cell Immunoglobulin and Mucin 3. Iran J Biotechnol. 2017 Sep 27;15(3):166-171  [Content Brief]

  [2]. Kim JS, et al. T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line. Immune Netw. 2012 Oct;12(5):207-12.  [Content Brief]

  [3]. Gandhi AK, et al. High resolution X-ray and NMR structural study of human T-cell immunoglobulin and mucindomain containing protein-3. Sci Rep. 2018 Nov 30;8(1):17512.  [Content Brief]