TMEM106B Protein, Mouse (HEK293, Fc)

The TMEM106B protein is a key player in neurons involved in trafficking to late endosomes/lysosomes and potentially affects dendritic morphogenesis and maintenance by regulating lysosomal trafficking. It may act as a molecular brake on retrograde transport by interacting with MAP6. TMEM106B Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TMEM106B protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TMEM106B Protein, Mouse (HEK293, Fc) is 157 a.a., with molecular weight of 65-68 kDa.

In neurons, the TMEM106B protein assumes a multifaceted role, participating in the transport of late endosomes/lysosomes and potentially influencing dendrite morphogenesis and maintenance by regulating lysosomal trafficking. It may act as a molecular brake for retrograde transport of late endosomes/lysosomes, possibly through its interaction with MAP6. Specifically in motoneurons, TMEM106B is implicated in mediating the axonal transport of lysosomes and axonal sorting at the initial segment. The exact directionality of its impact on the transport of moving lysosomes in neurites and its significance in lysosomal sorting within axons or dendrites remain unclear. Additionally, TMEM106B may play a role in the regulation of lysosomal size and responsiveness to stress in neurons, and it is essential for proper lysosomal acidification. The protein can form homomers and interacts with MAP6, vacuolar-type ATPase subunit ATP6AP1, AP2M1, CLTC, and TMEM106C, further highlighting its diverse molecular interactions in cellular processes.

Mouse

HEK293

C-hFc

Q80X71 (P119-Q275)

71900  [NCBI]

65-68 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.