TMPRSS11D Protein, Human (HEK293, His)

The TMPRSS11D protein is associated with host mucosal defense and acts synergistically with substances in the airway mucosa. It critically processes ACE2, activating the human coronavirus 229E spike glycoprotein to achieve virus-cell membrane fusion. TMPRSS11D Protein, Human (HEK293, His) is the recombinant human-derived TMPRSS11D protein, expressed by HEK293 , with N-His labeled tag. The total length of TMPRSS11D Protein, Human (HEK293, His) is 232 a.a., with molecular weight of ~27.6 kDa.

TMPRSS11D Protein is implicated in potential biological roles within the host defense system on the mucous membrane, either independently or in collaboration with other substances in airway mucous or bronchial secretions. Notably, it plays a key role in the proteolytic processing of ACE2, contributing to the activation of the human coronavirus 229E (HCoV-229E) spike glycoprotein. This activation facilitates virus-cell membrane fusions, a crucial step in viral entry. TMPRSS11D's proteolytic activity on the spike glycoprotein involves cleaving the C-terminal side of arginine residues at the P1 position of specific peptides, with Boc-Phe-Ser-Arg-4-methylcoumaryl-7-amide being the most efficiently cleaved substrate. Its enzymatic activity is optimal at a pH of 8.6 with this substrate. The protein is strongly inhibited by diisopropyl fluorophosphate, leupeptin, antipain, aprotinin, and soybean trypsin inhibitor, while showing minimal inhibition by secretory leukocyte protease inhibitor at 10 microM. These findings suggest that TMPRSS11D plays a multifaceted role in host defense and viral entry processes, highlighting its potential significance in the modulation of mucosal immunity and viral infections.

Human

HEK293

N-His

O60235/NP_004253.1 (I187-I418)

9407  [NCBI]

ILGGTEAEEGSWPWQVSLRLNNAHHCGGSLINNMWILTAAHCFRSNSNPRDWIATSGISTTFPKLRMRVRNILIHNNYKSATHENDIALVRLENSVTFTKDIHSVCLPAATQNIPPGSTAYVTGWGAQEYAGHTVPELRQGQVRIISNDVCNAPHSYNGAILSGMLCAGVPQGGVDACQGDSGGPLVQEDSRRLWFIVGIVSWGDQCGLPDKPGVYTRVTAYLDWIRQQTGI

Approximately 27.6 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.