TPBG/5T4 Protein, Human (HEK293, hFc)

TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This means that TPBG/5T4 plays a crucial role in regulating the complex Wnt/β-catenin signaling pathway and exerts a regulatory influence on cellular responses related to this pathway. TPBG/5T4 Protein, Human (HEK293, hFc) is the recombinant human-derived TPBG/5T4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TPBG/5T4 Protein, Human (HEK293, hFc) is 324 a.a., with molecular weight of 75-105 kDa.

The TPBG/5T4 protein appears to serve as an inhibitor of Wnt/beta-catenin signaling, potentially achieved through indirect interaction with LRP6, thereby impeding Wnt3a-dependent LRP6 internalization. This implies a crucial role for TPBG/5T4 in modulating the intricate Wnt/beta-catenin signaling pathway, exerting regulatory influence on cellular responses associated with this pathway. Further exploration of the specific molecular mechanisms governing the interaction between TPBG/5T4 and LRP6, as well as its impact on Wnt3a-dependent LRP6 internalization, could yield valuable insights into the functional significance of TPBG/5T4 in shaping Wnt/beta-catenin signaling dynamics and its potential implications for various cellular processes.

Immobilized Human TPBG, hFc Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC₅₀ of ≤30 ng/mL determined by ELISA.

Human

HEK293

C-hFc

Q13641 (S32-S355)

7162  [NCBI]

75-105 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.