TrkA Protein, Rat (HEK293, His)

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, His) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-His labeled tag. The total length of TrkA Protein, Rat (HEK293, His) is 386 a.a., with molecular weight of ~70-95 kDa.

The TrkA protein, a receptor tyrosine kinase, plays a vital role in the development and maturation of the central and peripheral nervous systems by regulating the proliferation, differentiation, and survival of sympathetic and sensory neurons. Serving as a high-affinity receptor for NGF, its primary ligand, TrkA can also be activated by NTF3/neurotrophin-3, though NTF3 specifically supports axonal extension through NTRK1 without influencing neuron survival. Upon dimeric NGF ligand-binding, TrkA undergoes homodimerization, autophosphorylation, and activation, subsequently recruiting, phosphorylating, and/or activating downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1. These effectors regulate distinct yet overlapping signaling cascades, steering cell survival and differentiation. Through SHC1 and FRS2, TrkA activates a GRB2-Ras-MAPK cascade controlling cell differentiation and survival, while through PLCG1, it modulates NF-Kappa-B activation and the transcription of genes crucial for cell survival. Additionally, through SHC1 and SH2B1, TrkA controls a Ras-PI3 kinase-AKT1 signaling cascade, further contributing to the regulation of cell survival. In the absence of ligand and activation, TrkA may promote cell death, underscoring the dependence of neuron survival on trophic factors.

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF, corresponding to a specific activity is 7.564×10^4 U/mg.

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  Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF,corresponding to a specific activity is 7.564×10⁴ U/mg

Rat

HEK293

C-His

P35739 (A33-P418)

59109  [NCBI]

AASCRETCCPVGPSGLRCTRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKIQMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLGLTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHHWCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSRDPVEKKDETP

Approximately 70-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.