TRMT112 Protein, Human (His-SUMO)

TRMT112 protein activates various methyltransferases for rRNA, tRNA, and protein modifications. It cooperates with BUD23 to methylate guanine N(7) of 18S rRNA. TRMT112 Protein, Human (His-SUMO) is the recombinant human-derived TRMT112 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

TRMT112 protein serves as an activator for a diverse range of methyltransferases involved in rRNA, tRNA, and protein modifications. Teaming up with methyltransferase BUD23, it methylates the N(7) position of a guanine in 18S rRNA, while in collaboration with N6AMT1/HEMK2, it catalyzes N5-methylation of ETF1 on 'Gln-185' and monomethylates 'Lys-12' of histone H4 (H4K12me1). Additionally, in conjunction with ALKBH8, TRMT112 participates in the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in specific tRNA species. Partnering with methyltransferase THUMPD3, it contributes to the formation of N(2)-methylguanosine in various tRNA substrates. Furthermore, TRMT112, along with METTL5, plays a crucial role in methylating the 6th position of adenine in position 1832 of 18S rRNA. Its involvement in pre-rRNA processing contributes to small-subunit rRNA production, and the formation of various heterodimers with BUD23, N6AMT1/HEMK2, ALKBH8, and METTL5 highlights its diverse regulatory functions in various methylation pathways. Interactions with THUMPD3, THUMPD2, and TRMT11 further underscore its intricate role in these processes.

Human

E. coli

N-His;N-SUMO

Q9UI30-1 (M1-S125)

51504  [NCBI]

MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADNLRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES

Approximately 32 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.