UBE2I Protein, Human (GST)

UBE2I Protein, a key participant in protein sumoylation, accepts ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Teaming up with E3 ligases like RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins, including critical substrates FOXL2 and KAT5. Its enzymatic activity extends to forming poly-SUMO chains, influencing diverse cellular processes and playing a pivotal role in specific protein modifications, such as sumoylation of p53/TP53 at 'Lys-386' and ERCC6. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2I Protein, Human (GST) is 158 a.a., with molecular weight of ~40.0 kDa.

UBE2I, a crucial player in the protein sumoylation process, demonstrates versatility by accepting ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Collaborating with various E3 ligases, such as RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins. This enzymatic activity extends to the formation of poly-SUMO chains, emphasizing its role in diverse cellular processes. UBE2I is essential for sumoylation events involving critical substrates like FOXL2 and KAT5, contributing to nuclear architecture and chromosome segregation. Furthermore, UBE2I-mediated sumoylation plays a pivotal role in specific protein modifications, including sumoylation of p53/TP53 at 'Lys-386' and ERCC6, the latter being indispensable for its transcription-coupled nucleotide excision repair activity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P63279 (M1-S158)

7329  [NCBI]

MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.