UMP-CMP kinase/CMPK1 Protein, Human (sf9, His)

The UMP-CMP kinase (CMPK1) protein utilizes ATP as a phosphate donor to catalyze the phosphorylation of pyrimidine nucleoside monophosphate, thereby playing a key role in cellular processes. This enzymatic activity is integral to the de novo biosynthesis of pyrimidine nucleotides and contributes to the synthesis of important cellular building blocks. UMP-CMP kinase/CMPK1 Protein, Human (sf9, His) is the recombinant human-derived UMP-CMP kinase/CMPK1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

The UMP-CMP kinase, also known as CMPK1, is a crucial enzyme that catalyzes the phosphorylation of pyrimidine nucleoside monophosphates utilizing ATP as a phosphate donor. This enzymatic activity holds significance in de novo pyrimidine nucleotide biosynthesis, representing a key step in the synthesis of essential cellular components. CMPK1 exhibits a preference for uridine monophosphate (UMP) and cytidine monophosphate (CMP) as phosphate acceptors in this process. Additionally, CMPK1 displays broad nucleoside diphosphate kinase activity, emphasizing its versatility in catalyzing the transfer of phosphate groups between different nucleoside diphosphates. The multifaceted functions of CMPK1 underscore its pivotal role in cellular nucleotide metabolism and its potential implications for various cellular processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P30085-1 (M1-G196)

51727  [NCBI]

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM Nacl, pH 7.4, 10% glycerol

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.