VCP Protein, Human (His)

VCP proteins are indispensable for Golgi stack dynamics, coordinating fragmentation during mitosis and postmitotic reorganization. It actively promotes the formation of the transitional endoplasmic reticulum (tER), enables ATP-dependent vesicle budding, and enables membrane transfer to the Golgi apparatus. VCP Protein, Human (His) is the recombinant human-derived VCP protein, expressed by E. coli , with C-6*His labeled tag. The total length of VCP Protein, Human (His) is 588 a.a., with molecular weight of ~75.0 kDa.

VCP protein is essential for orchestrating the dynamic processes associated with Golgi stacks, playing a crucial role in their fragmentation during mitosis and subsequent reassembly post-mitosis. It actively participates in the formation of transitional endoplasmic reticulum (tER), facilitating the transfer of membranes from the endoplasmic reticulum to the Golgi apparatus through ATP-dependent vesicle budding from the tER. The VCP, UFD1, and NPLOC4 ternary complex is pivotal for binding ubiquitinated proteins and mediating the export of misfolded proteins from the endoplasmic reticulum to the cytoplasm for proteasomal degradation. Additionally, VCP is integral to various cellular processes, including the regulation of spindle disassembly, formation of a closed nuclear envelope, and modulation of E3 ubiquitin-protein ligase activity. It actively engages in endoplasmic reticulum stress-induced quality control and plays a crucial role in DNA damage response by participating in the repair of double-strand breaks and stalled replication forks. Furthermore, VCP is involved in the clearance process, mediating the extraction of stressed mitochondrial outer-membrane proteins and facilitating the maturation of ubiquitin-containing autophagosomes. Its multifaceted functions extend to negative regulation of type I interferon production, playing a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes, and indirectly regulating the insulin-like growth factor receptor signaling pathway by controlling the steady-state expression of the IGF1R receptor.

No Enzyme activity.

Human

E. coli

C-6*His

P55072 (A2-N589)

7415  [NCBI]

ASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGN

Approximately 69.0-75.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.