1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Cadherins
  5. Cadherin-5
  6. VE-Cadherin Protein, Human (HEK293, C-His-Fc)

VE-Cadherin Protein, Human (HEK293, C-His-Fc)

Cat. No.: HY-P73560A
COA Handling Instructions

The FGFR-4 protein binds to its ligands and activates signaling pathways involved in cell growth, differentiation, and survival. It plays a role in various developmental processes, including skeletal development, liver metabolism, and cancer progression. In the absence of ligand binding, FGFR-4 remains inactive and does not initiate downstream signaling cascades. VE-Cadherin Protein, Human (HEK293, C-His-Fc) is the recombinant human-derived VE-Cadherin protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of VE-Cadherin Protein, Human (HEK293, C-His-Fc) is 568 a.a., with molecular weight of ~125 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The FGFR-4 protein binds to its ligands and activates signaling pathways involved in cell growth, differentiation, and survival. It plays a role in various developmental processes, including skeletal development, liver metabolism, and cancer progression. In the absence of ligand binding, FGFR-4 remains inactive and does not initiate downstream signaling cascades. VE-Cadherin Protein, Human (HEK293, C-His-Fc) is the recombinant human-derived VE-Cadherin protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of VE-Cadherin Protein, Human (HEK293, C-His-Fc) is 568 a.a., with molecular weight of ~125 kDa.

Background

The VE-Cadherin Protein, belonging to the classical cadherin superfamily, encodes a preproprotein that undergoes proteolytic processing to yield the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule comprises five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. Operating as a classical cadherin, it confers upon cells the ability to adhere in a homophilic manner, thereby playing a pivotal role in the assembly and maintenance of endothelial adherens junctions. Located in a gene cluster on the long arm of chromosome 16, this gene is implicated in loss of heterozygosity events in breast and prostate cancer. Noteworthy is its biased expression, with elevated levels observed in the placenta (RPKM 108.8), lung (RPKM 57.5), and 12 other tissues, underscoring its potential significance across a spectrum of physiological contexts.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 59.22 ng/ml, corresponding to a specific activity is 1.689×10^4 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 59.22 ng/mL, corresponding to a specific activity is 1.689×104 units/mg.
Species

Human

Source

HEK293

Tag

C-hFc;C-6*His

Accession

NP_001786.2 (A26-Q593)

Gene ID
Molecular Construction
N-term
VE-Cadherin (A26-Q593)
Accession # NP_001786.2
hFc-6*His
C-term
Synonyms
Cadherin-5; VE-cadherin; CD144; CDH5
AA Sequence

AGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQ

Molecular Weight

Approximately 125 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

VE-Cadherin Protein, Human (HEK293, C-His-Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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VE-Cadherin Protein, Human (HEK293, C-His-Fc)
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HY-P73560A
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