Vitronectin Protein, Mouse (HEK293, His)

Vitronectin is a key cell adhesion factor in serum and tissues and plays a crucial role in cell interactions by binding to glycosaminoglycans and proteoglycans.It is recognized by specific integrins and serves as an important adhesion molecule, facilitating cell-matrix interactions.Vitronectin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vitronectin protein, expressed by HEK293 , with C-His labeled tag.

Vitronectin, a pivotal cell adhesion and spreading factor present in both serum and tissues, plays a crucial role in cellular interactions. It engages with glycosaminoglycans and proteoglycans, demonstrating its versatility in molecular interactions. Recognized by specific integrins, Vitronectin serves as a vital cell-to-substrate adhesion molecule, facilitating cellular adhesion processes. Additionally, it acts as an inhibitor, mitigating the membrane-damaging effects associated with the terminal cytolytic complement pathway. Notably, Vitronectin establishes connections with various molecules, including SERPINE1/PAI1, insulin, and C1QBP, showcasing its involvement in diverse cellular processes.

1.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37°C.
2.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (5 μg/mL and 100 μL/well), approximately is 79.89% adhered specifically after 30 minutes at 37°C.

Mouse

HEK293

C-His

P29788 (D20-K478)

22370  [NCBI]

DQESCKGRCTQGFMASKKCQCDELCTYYQSCCADYMEQCKPQVTRGDVFTMPEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLNPRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPDTTDQGTPEFPEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWEYEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHGVPGKVDAAMAGRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSRSIWFSLFSSEESGLGTYNNYDYDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK

Approximately 73-90 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or 20 mM PB, 500 mM NaCl , pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.