2. GPCR/G Protein Neuronal Signaling
  3. Neuropeptide Y Receptor
  4. RFRP-3(human)

RFRP-3(human)  (Synonyms: Neuropeptide VF(124-131)(human))

Cat. No.: HY-P1250 Purity: 99.21%
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RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+?mobilization. RFRP-3(human) is a NPFF1?receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM.

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RFRP-3(human) Chemical Structure

RFRP-3(human) Chemical Structure

CAS No. : 311309-27-0

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10 mg USD 780 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of RFRP-3(human):

RFRP-3(human) Related Antibodies

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NPY Y1 receptor NPY Y2 receptor NPY Y4 receptor NPY Y5 receptor

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Description

RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+?mobilization. RFRP-3(human) is a NPFF1?receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].

Cellular Effect
Cell Line Type Value Description References
COS-1 EC50
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
[PMID: 22708927]
COS-1 EC50
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
[PMID: 22708927]
In Vitro

RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].
Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM)[1].
RFRP-3 (10-8?to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RFRP-3(human) Related Antibodies
Molecular Weight

969.14

Formula

C45H72N14O10
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

VPNLPQRF-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (103.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0318 mL 5.1592 mL 10.3184 mL
5 mM 0.2064 mL 1.0318 mL 2.0637 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0318 mL 5.1592 mL 10.3184 mL 25.7961 mL
5 mM 0.2064 mL 1.0318 mL 2.0637 mL 5.1592 mL
10 mM 0.1032 mL 0.5159 mL 1.0318 mL 2.5796 mL
15 mM 0.0688 mL 0.3439 mL 0.6879 mL 1.7197 mL
20 mM 0.0516 mL 0.2580 mL 0.5159 mL 1.2898 mL
25 mM 0.0413 mL 0.2064 mL 0.4127 mL 1.0318 mL
30 mM 0.0344 mL 0.1720 mL 0.3439 mL 0.8599 mL
40 mM 0.0258 mL 0.1290 mL 0.2580 mL 0.6449 mL
50 mM 0.0206 mL 0.1032 mL 0.2064 mL 0.5159 mL
60 mM 0.0172 mL 0.0860 mL 0.1720 mL 0.4299 mL
80 mM 0.0129 mL 0.0645 mL 0.1290 mL 0.3225 mL
100 mM 0.0103 mL 0.0516 mL 0.1032 mL 0.2580 mL
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RFRP-3(human) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RFRP-3(human)311309-27-0Neuropeptide VF(124-131)(human)Neuropeptide Y ReceptorNPY receptorHypothalamicPituitaryAxisLHFSHGnIHhomologInhibitorinhibitorinhibit

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