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  4. Rhodamine B isothiocyanate

Rhodamine B isothiocyanate (RBITC) is a fluorescent dye commonly used in biological imaging and labeling applications. It has an isothiocyanate functional group that can be covalently attached to amino groups on proteins and other biomolecules, making it useful for fluorescent labeling of cells and tissues. RBITC fluoresces bright orange-red under green light excitation, which makes it easy to detect and track labeled molecules in complex samples. Due to its stability and sensitivity, RBITC has been widely used in various research fields, including cell biology, immunology, and neurobiology.

For research use only. We do not sell to patients.

Rhodamine B isothiocyanate Chemical Structure

Rhodamine B isothiocyanate Chemical Structure

CAS No. : 36877-69-7

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Rhodamine B isothiocyanate Related Antibodies

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  • Purity & Documentation

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Description

Rhodamine B isothiocyanate (RBITC) is a fluorescent dye commonly used in biological imaging and labeling applications. It has an isothiocyanate functional group that can be covalently attached to amino groups on proteins and other biomolecules, making it useful for fluorescent labeling of cells and tissues. RBITC fluoresces bright orange-red under green light excitation, which makes it easy to detect and track labeled molecules in complex samples. Due to its stability and sensitivity, RBITC has been widely used in various research fields, including cell biology, immunology, and neurobiology.

In Vitro

Rhodamine B isothiocyanate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine B isothiocyanate Related Antibodies
Molecular Weight

536.08

Formula

C29H30ClN3O3S
CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

CCN(C1=CC2=C(C(C3=C(N=C=S)C=CC=C3C(O)=O)=C4C=C/C(C=C4O2)=[N+](CC)\CC)C=C1)CC.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (23.32 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8654 mL 9.3270 mL 18.6539 mL
5 mM 0.3731 mL 1.8654 mL 3.7308 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 97.97%

SDS (558 KB)

COA (257 KB) HNMR (278 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8654 mL 9.3270 mL 18.6539 mL 46.6348 mL
5 mM 0.3731 mL 1.8654 mL 3.7308 mL 9.3270 mL
10 mM 0.1865 mL 0.9327 mL 1.8654 mL 4.6635 mL
15 mM 0.1244 mL 0.6218 mL 1.2436 mL 3.1090 mL
20 mM 0.0933 mL 0.4663 mL 0.9327 mL 2.3317 mL
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Rhodamine B isothiocyanate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine B isothiocyanate36877-69-7Biochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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