RUSKI-201

Cat. No.: HY-123781: FAQs
Data Sheet Handling Instructions

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RUSKI-201 is a potent and specific Hedgehog acyltransferase (Hhat) inhibitor, with an IC₅₀ of 0.20 μM. RUSKI-201 is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function.

For research use only. We do not sell to patients.

RUSKI-201 Chemical Structure

CAS No. : 1458031-48-5

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MOQ		Minimum order quantity
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Other Forms of RUSKI-201:

RUSKI-201 dihydrochloride Get quote

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.2 μM Compound: 3; RU-SKI201	Inhibition of 3C-FLAG/His8-tagged human HHAT expressed in HEK293 cells assessed as reduction in enzyme-mediated sonic hedgehog (1 to 11 residues) palmitoylation by click-ELISA method Inhibition of 3C-FLAG/His8-tagged human HHAT expressed in HEK293 cells assessed as reduction in enzyme-mediated sonic hedgehog (1 to 11 residues) palmitoylation by click-ELISA method	[PMID: 30986059]

Molecular Weight

357.51

Formula

C₂₀H₂₇N₃OS

CAS No.

1458031-48-5

SMILES

CCC(C)CNCC(N1CCC2=C(C=CS2)C1C3=NC(C)=CC=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (279.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7971 mL	13.9856 mL	27.9712 mL
5 mM	0.5594 mL	2.7971 mL	5.5942 mL
10 mM	0.2797 mL	1.3986 mL	2.7971 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (6.99 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Ursula R Rodgers, et al. Characterization of Hedgehog Acyltransferase Inhibitors Identifies a Small Molecule Probe for Hedgehog Signaling by Cancer Cells. ACS Chem Biol. 2016 Dec 16;11(12):3256-3262. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7971 mL	13.9856 mL	27.9712 mL	69.9281 mL
	5 mM	0.5594 mL	2.7971 mL	5.5942 mL	13.9856 mL
	10 mM	0.2797 mL	1.3986 mL	2.7971 mL	6.9928 mL
	15 mM	0.1865 mL	0.9324 mL	1.8647 mL	4.6619 mL
	20 mM	0.1399 mL	0.6993 mL	1.3986 mL	3.4964 mL
	25 mM	0.1119 mL	0.5594 mL	1.1188 mL	2.7971 mL
	30 mM	0.0932 mL	0.4662 mL	0.9324 mL	2.3309 mL
	40 mM	0.0699 mL	0.3496 mL	0.6993 mL	1.7482 mL
	50 mM	0.0559 mL	0.2797 mL	0.5594 mL	1.3986 mL
	60 mM	0.0466 mL	0.2331 mL	0.4662 mL	1.1655 mL
	80 mM	0.0350 mL	0.1748 mL	0.3496 mL	0.8741 mL
	100 mM	0.0280 mL	0.1399 mL	0.2797 mL	0.6993 mL

RUSKI-201 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RUSKI-2011458031-48-5RUSKI201RUSKI 201HedgehogHhatHh palmitoylationH520Panc-1MCF-7Shh-Light2tumorInhibitorinhibitorinhibit

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