2. GPCR/G Protein
  3. EBI2/GPR183
  4. SAE-14

SAE-14  (Synonyms: GPR183 antagonist-1)

Cat. No.: HY-147222 Purity: 98.26%
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SAE-14 (compound SAE-14) is a potent, specific GPR183 antagonist with an IC50 value of 28.5 nM, can antagonize 7α, 25-OHC–induced calcium mobilization with IC50 value below 50 nM in HL-60 cells. GPR183 antagonist-1 can reverse allodynia in mice.

For research use only. We do not sell to patients.

SAE-14 Chemical Structure

SAE-14 Chemical Structure

CAS No. : 1241280-25-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 192 In-stock
Solution
10 mM * 1 mL in DMSO USD 192 In-stock
Solid
5 mg USD 175 In-stock
10 mg USD 280 In-stock
25 mg USD 480 In-stock
50 mg USD 700 In-stock
100 mg USD 1000 In-stock
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Based on 1 publication(s) in Google Scholar

SAE-14 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SAE-14

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SAE-14 (compound SAE-14) is a potent, specific GPR183 antagonist with an IC50 value of 28.5 nM, can antagonize 7α, 25-OHC–induced calcium mobilization with IC50 value below 50 nM in HL-60 cells. GPR183 antagonist-1 can reverse allodynia in mice[1].

IC50 & Target

IC50: 28.5 nM (GPR183)[1]
In Vitro

SAE-14 (compound SAE-14) can able to antagonize 7α, 25-OHC–induced calcium mobilization with an IC50 value below 50 nM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SAE-14 Related Antibodies

Cell Viability Assay[1]

Cell Line: The human leukemia (HL)-60 cells
Concentration: 5×of the antagonist
Incubation Time: 15 min
Result: Had GPR183-specific (IC50: 28.5nM) and abolished 7a, 25-OHC–induced calcium mobilization in the HL-60 cells.
In Vivo

SAE-14 (compound SAE-14) (i.th.; 2.9 µM; once) can reverse nerve injury-induced allodynia in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male and female ICR mice[1]
Dosage: 2.9 µM
Administration: intrathecal (i.th.) injections; 2.9 µM; once
Result: Reversed CCI-induced mechanical allodynia in a time-dependent manner.
Molecular Weight

364.36

Formula

C19H19F3N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(N2CCOCC2)=CC(F)=C1)CCC3=CC=C(F)C(F)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (274.45 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7445 mL 13.7227 mL 27.4454 mL
5 mM 0.5489 mL 2.7445 mL 5.4891 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7445 mL 13.7227 mL 27.4454 mL 68.6135 mL
5 mM 0.5489 mL 2.7445 mL 5.4891 mL 13.7227 mL
10 mM 0.2745 mL 1.3723 mL 2.7445 mL 6.8613 mL
15 mM 0.1830 mL 0.9148 mL 1.8297 mL 4.5742 mL
20 mM 0.1372 mL 0.6861 mL 1.3723 mL 3.4307 mL
25 mM 0.1098 mL 0.5489 mL 1.0978 mL 2.7445 mL
30 mM 0.0915 mL 0.4574 mL 0.9148 mL 2.2871 mL
40 mM 0.0686 mL 0.3431 mL 0.6861 mL 1.7153 mL
50 mM 0.0549 mL 0.2745 mL 0.5489 mL 1.3723 mL
60 mM 0.0457 mL 0.2287 mL 0.4574 mL 1.1436 mL
80 mM 0.0343 mL 0.1715 mL 0.3431 mL 0.8577 mL
100 mM 0.0274 mL 0.1372 mL 0.2745 mL 0.6861 mL
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SAE-14 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SAE-141241280-25-0GPR183 antagonist-1SAE14SAE 14EBI2/GPR183GPR183 antagonist SAE-14HL-60Inhibitorinhibitorinhibit

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