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Results for "

enzymatic hydrolysis

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Adrenergic Receptor (1) Amino Acid Derivatives (1) Angiotensin-converting Enzyme (ACE) (1) Bacterial (2) Biochemical Assay Reagents (2) Endogenous Metabolite (4) Fluorescent Dye (3) Glycosidase (1) HDAC (1) PPAR (1)
By Research Areas:	Cancer (4) Cardiovascular Disease (1) Infection (2) Inflammation/Immunology (1) Metabolic Disease (2)

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Natural
Products

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-E70130

Snailase, Snail gastrointestinal	Others	Others
Snailase, Snail gastrointestinal is an enzyme mixture composed of more than 20 enzymes, which is often used for enzymatic hydrolysis of purified flavonoid glycosides. Snailase can be obtained from the digestive tract and includes cellulase, sucrase, hemicellulase, pectinase, polygalacturonase, protease, etc .

HY-129217

Naringinase	Glycosidase	Others
Naringinase, a hydrolytic enzymatic complex, possesses the activity of both α-L-rhamnosidase and β-D-glucosidase. Naringinase has wide occurrence in nature. Naringinase can be used in the biotransformation of steroids, antibiotics, and mainly on glycosides hydrolysis .

HY-P2893

Creatinase, Actinobacteria Creatine amidohydrolase	Endogenous Metabolite	Others
Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration .

HY-N6821

2-O-α-D-Glucopyranosyl-L-ascorbic Acid	Others	Cancer
2-O-α-D-Glucopyranosyl-L-ascorbic Acid is a glucoside derivative of ascorbic acid, shows anti-cancer activity after enzymatic hydrolysis to ascorbic acid .

HY-N3169

Nyasicol (+)-Nyasicol	Others	Others
Nyasicol ((+)-Nyasicol) is an enzymatic (β-glucosidase) hydrolysis product of Compound 3 .

HY-E70101

α-Glucuronidase	Others	Others
α-Glucuronidase plays an essential role in the full enzymatic hydrolysis of hemicellulose. α-Glucuronidase cleaves the attached MeGlcA moiety from the xylose .

HY-19602

Cedefingol	Others	Cancer
Cedefingol is an antitumor compound with α-glucosidase inhibitory activity. Cedefingol was identified together with other compounds in fermented dark tea samples. The biotransformation of cedefingol is related to enzymatic hydrolysis, hydrolysis and biosynthesis. Cedefingol exhibited different bioactivities in samples with different fermentation times. The antioxidant properties of cedefingol showed certain activity in the DPPH model .

HY-129683

AM3102	PPAR	Metabolic Disease
AM3102 is an oleoylethanolamide (OEA) analog. AM3102 is an endogenous high-affinity PPAR-alpha agonist. AM3102 resists enzymatic hydrolysis, activates PPAR-alpha with high potency in vitro, and persistently reduces feeding when administered in vivo either parenterally or orally .

HY-W008919

Boc-Lys(Boc)-Onp N-Alpha,N-epsilon-di-Boc-L-lysine 4-nitrophenyl ester	Amino Acid Derivatives	Others
Boc-Lys(Boc)-Onp (N-Alpha,N-epsilon-di-Boc-L-lysine 4-nitrophenyl ester) is a lysine with a Boc protecting group. Boc-Lys(Boc)-Onp was used as a substrate for a catalyst model to study its enzymatic hydrolysis reaction catalyzed by a copper(II) complex .

HY-P2979A

Invertase, candida sp. β-D-Fructofuranosidase, candida sp.	Biochemical Assay Reagents	Others
Invertase from Candida sp. is responsible for catalyzing the hydrolysis of sucrose into glucose and fructose and is widely used in the field of carbohydrate processing. Invertase from Candida sp. can be used for enzymatic determination of sucrose concentration as well as for structural analysis of carbohydrates containing β-D-fructofuranosyl residues .

HY-W099563

4-Nitrophenyl stearate	Biochemical Assay Reagents	Others
4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

HY-P2797A

Chitinase, Streptomyces griseus AMCase, Streptomyces griseus	Endogenous Metabolite	Others
Chitinase, Streptomyces griseus is a chitinase mainly found in Bacillus species and uses colloidal chitin as a substrate. The Km and Vmax of Chitinase are 4.28 mg/mL and 14.29 μg/(Min·mL) respectively. The final enzymatic hydrolysis product is N-acetylglucosamine, and the monomer and dimer contents are 14.33% and 85.67% respectively .

HY-137798

Chromozym PL	Fluorescent Dye	Others
Chromozym PL is a chromogenic substrate for plasmin, and the enzymatic reaction can be carried out in 0.1mL Tris-HCl buffer (50 mM, pH 7.8). 100 μM Chromozym PL was dissolved and prepared. After adding the hydrolase, the generation of p-nitroaniline (pNA) at 405 nm was continuously observed, and the hydrolysis products were calculated .

HY-N6821R

2-O-α-D-Glucopyranosyl-L-ascorbic Acid (Standard)	Others	Cancer
2-O-α-D-Glucopyranosyl-L-ascorbic Acid (Standard) is the analytical standard of 2-O-α-D-Glucopyranosyl-L-ascorbic Acid. This product is intended for research and analytical applications. 2-O-α-D-Glucopyranosyl-L-ascorbic Acid is a glucoside derivative of ascorbic acid, shows anti-cancer activity after enzymatic hydrolysis to ascorbic acid .

HY-P2893A

Creatinase (Recombinant) Creatine amidinohydrolase	Endogenous Metabolite	Metabolic Disease
Creatinase (Recombinant) is a hydrolase responsible for catalyzing the hydrolysis of creatine (HY-W010388) into sarcosine (HY-101037) and urea (HY-Y0271). Creatinase (Recombinant) can be used in conjunction with creatinine deiminase (HY-P2838) and sarcosine oxidase (HY-P2861) in an enzyme cascade reaction to measure creatinine (HY-B0504) levels in blood and urine. Creatinase (Recombinant) is the rate-limiting enzyme in this enzymatic cascade, and the enzymatic quantification of creatinine is an important method for evaluating kidney function .

HY-N7538

Dealanylalahopcin	Bacterial	Infection
Dealanylalahopcin is an antimicrobial agent that can be isolated from the culture filtrate of Streptomyces leucoderma subsp. ochraceus. Dealanylalahopcin can also be synthesized by enzymatic hydrolysis of alahopcin by microbial α-amino acid ester hydrolases. Dealanylalahopcin has weak antimicrobial activity against Gram-positive and Gram-negative bacteria and exhibits moderate inhibition of collagen prolyl hydroxylase .

HY-W154295

Purple-β-D-Gal	Fluorescent Dye	Others
Purple-β-D-Gal is a chromogenic β-galactosidase substrate. Intracellular enzymatic hydrolysis of Purple-β-D-Gal generates free indoxyl molecules, which undergo in situ oxidation and subsequent dimerization to produce chromogenic, water-insoluble, indigo precipitates. Purple-β-D-Gal can be used for the detection of β-galactosidase activity .

HY-W013168

4-Nitrophenyl palmitate 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp	Bacterial	Infection
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate) is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-Nitrophenyl palmitate, 4-nitrophenol is released, which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipase.

HY-105572

Moveltipril calcium MC-838 calcium	Angiotensin-converting Enzyme (ACE)	Cardiovascular Disease
Moveltipril calcium (MC-838 calcium) is an orally active angiotensin converting enzyme (ACE) inhibitor with antihypertensive activity. Moveltipril calcium binds via a stable thioester bond and exhibits relative resistance to enzymatic hydrolysis in rat liver homogenate. Moveltipril calcium effectively inhibits ACE extracted from rabbit lung in a concentration-dependent manner. Moveltipril calcium is able to highly specifically inhibit the contractile response to angiotensin-I (AI) in free rat aortic rings and guinea pig ileum preparations, while enhancing the contractile response to calcitonin .

HY-138111

4-Nitrophenyl β-D-cellotetraoside p-Nitopheyl β-D-cellotetaoside	Fluorescent Dye	Others
4-Nitrophenyl β-D-cellotetraoside (p-Nitopheyl β-D-cellotetaoside) is a small molecule cellulose mimetic consisting of a tetramer of D-glucose units linked by β-1-4 glycosidic bonds. The fragmentation pattern of 4-Nitrophenyl β-D-cellotetraoside after enzymatic hydrolysis can be analyzed by TLC or by the release of 4-nitrophenol, which has a strong absorbance at 395 nm in alkaline solutions. 4-Nitrophenyl β-D-cellotetraoside can be used in cellulose degradation studies to determine the specificity of cellulases .

HY-135115

Oleuropein Aglycone 3,4-DHPEA-EA	HDAC Adrenergic Receptor	Inflammation/Immunology
Oleuropein Aglycone (3,4-DHPEA-EA) is a polyphenol and the aglycone form of oleuropein (HY-N0292), formed by enzymatic, acidic or acetylated hydrolysis of oleuropein. Dietary intake of oleuropein Aglycone (50 mg/kg diet) increases the number of neuronal autophagic vesicles, reverses cognitive deficits in the TgCRND8 transgenic mouse model of Alzheimer's disease, and reduces the levels of histone deacetylase 2 (HDAC2) in the cortex and hippocampus. Oleuropein Aglycone increases urinary norepinephrine, interscapular brown adipose tissue epinephrine, and UCP1 protein levels, and reduced plasma leptin levels and total abdominal adipose tissue weight in a rat model of high-fat diet-induced obesity. Oleuropein Aglycone also reduced lung neutrophil infiltration, lipid peroxidation, and IL-1β levels in a mouse model of carrageenan-induced pleurisy.

HY-113225B

Guanosine triphosphate tritris GTP tritris	Endogenous Metabolite	Cancer
Guanosine triphosphate tritris (GTP tritris) serves as a vital enhancer of myogenic cell differentiation and plays a critical role in modulating miRNA-myogenic regulator factors. It also facilitates the release of exosomes enriched with guanosine and guanosine-derived molecules, and is regarded as an activated precursor for RNA synthesis. In mitochondrial function, GTP participates in the import of proteins into the matrix, which is essential for various regulated pathways, and is involved in initiating peptide synthesis through the binding of formylmethionyl-tRNA to the ribosome, as well as polypeptide chain elongation. Additionally, GTP acts as a phosphate and pyrophosphate carrier that channels chemical energy into specific biosynthetic pathways. It activates signal transducing G proteins that regulate cellular processes such as proliferation and differentiation, and its hydrolysis by small GTPases, including Ras and Rho, is integral to both proliferation and apoptosis. Furthermore, the small GTPase Rab is instrumental in vesicle docking, fusion, and formation. Beyond signal transduction, GTP is an energy-rich precursor in the enzymatic biosynthesis of DNA and RNA.

Cat. No.	Product Name	Type

HY-W154295

Purple-β-D-Gal	Fluorescent Dyes/Probes
Purple-β-D-Gal is a chromogenic β-galactosidase substrate. Intracellular enzymatic hydrolysis of Purple-β-D-Gal generates free indoxyl molecules, which undergo in situ oxidation and subsequent dimerization to produce chromogenic, water-insoluble, indigo precipitates. Purple-β-D-Gal can be used for the detection of β-galactosidase activity .

HY-W013168

4-Nitrophenyl palmitate 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp	Dyes
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate) is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-Nitrophenyl palmitate, 4-nitrophenol is released, which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipase.

4-Nitrophenyl palmitate

4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp

Dyes

4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate) is a colorimetric lipase and esterase substrate. Upon enzymatic hydrolysis of 4-Nitrophenyl palmitate, 4-nitrophenol is released, which can be quantified by colorimetric detection at 410 nm as a measure of enzyme activity. 4-Nitrophenyl palmitate has been used to characterize the activity of various bacterial and mammalian enzymes, including Burkholderia and porcine pancreatic lipase.

HY-138111

4-Nitrophenyl β-D-cellotetraoside p-Nitopheyl β-D-cellotetaoside	Fluorescent Dyes/Probes
4-Nitrophenyl β-D-cellotetraoside (p-Nitopheyl β-D-cellotetaoside) is a small molecule cellulose mimetic consisting of a tetramer of D-glucose units linked by β-1-4 glycosidic bonds. The fragmentation pattern of 4-Nitrophenyl β-D-cellotetraoside after enzymatic hydrolysis can be analyzed by TLC or by the release of 4-nitrophenol, which has a strong absorbance at 395 nm in alkaline solutions. 4-Nitrophenyl β-D-cellotetraoside can be used in cellulose degradation studies to determine the specificity of cellulases .

4-Nitrophenyl β-D-cellotetraoside

p-Nitopheyl β-D-cellotetaoside

Fluorescent Dyes/Probes

4-Nitrophenyl β-D-cellotetraoside (p-Nitopheyl β-D-cellotetaoside) is a small molecule cellulose mimetic consisting of a tetramer of D-glucose units linked by β-1-4 glycosidic bonds. The fragmentation pattern of 4-Nitrophenyl β-D-cellotetraoside after enzymatic hydrolysis can be analyzed by TLC or by the release of 4-nitrophenol, which has a strong absorbance at 395 nm in alkaline solutions. 4-Nitrophenyl β-D-cellotetraoside can be used in cellulose degradation studies to determine the specificity of cellulases .

Cat. No.	Product Name	Type

HY-129217

Naringinase	Biochemical Assay Reagents
Naringinase, a hydrolytic enzymatic complex, possesses the activity of both α-L-rhamnosidase and β-D-glucosidase. Naringinase has wide occurrence in nature. Naringinase can be used in the biotransformation of steroids, antibiotics, and mainly on glycosides hydrolysis .

HY-W099563

4-Nitrophenyl stearate	Enzyme Substrates
4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

4-Nitrophenyl stearate

Enzyme Substrates

4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

Cat. No.	Product Name	Target	Research Area