2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Sialyl-Lewis X

Sialyl-Lewis X (sLeX) is a sialylated fucosylated tetrasaccharide, an endogenous antigen. Sialyl-Lewis X is a high-affinity ligand for selectins (E-, P-, and L-selectin). Sialyl-Lewis X binds to ELAM-1 and CD62 and has the ability?to inhibits CD62-mediated neutrophil recruitment to sites of inflammation.

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Sialyl-Lewis X Chemical Structure

Sialyl-Lewis X Chemical Structure

CAS No. : 98603-84-0

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Based on 1 publication(s) in Google Scholar

Sialyl-Lewis X Related Antibodies

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Description

Sialyl-Lewis X (sLeX) is a sialylated fucosylated tetrasaccharide, an endogenous antigen. Sialyl-Lewis X is a high-affinity ligand for selectins (E-, P-, and L-selectin)[1]. Sialyl-Lewis X binds to ELAM-1 and CD62 and has the ability?to inhibits CD62-mediated neutrophil recruitment to sites of inflammation[2].
IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
HL-60 IC50
3.3 mM
Compound: sLex
Tested in vitro for the concentration to inhibit sLex bearing HL-60 cells binding to Selectin E-IgG fusion proteins by 50%.
Tested in vitro for the concentration to inhibit sLex bearing HL-60 cells binding to Selectin E-IgG fusion proteins by 50%.
[PMID: 9544210]
In Vitro

Sialyl-Lewis X is a high-affinity ligand of CD62, Antibodies [mAb CSLEX (IgM; anti-sLeX)] to sLeX inhibit CD62-mediated binding of HL-60 cells to activated platelets[1].
Liposomes containing glycolipids with the sLeX structure prevents adhesion of HL-60 cells and human neutrophils. HL-60 cell adhesion is partially inhibited (50%) by liposomes containing Lex at 5 μg/ml. However, sLeX liposomes give maximal inhibition at only 1 ug/ml. sLeX liposomes inhibits adhesion with a 10-fold higher affinity than Lex liposomes[1].
CD62 binding of neutrophils to activated platelets is inhibited a soluble human milk oligosaccharide that contains the LeX structure. the sLeX sugar is a 30-fold more potent inhibitor than the nonsialylated Lex sugar, which requires 2 μg/ml and 54 μg/ml to achieve 50% inhibition of neutrophil adhesion, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sialyl-Lewis X Related Antibodies
Molecular Weight

820.74

Formula

C31H52N2O23
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC([C@](O[C@@]([C@@H]1NC(C)=O)([H])[C@H](O)[C@H](O)CO)(C[C@@H]1O)O[C@@H]([C@H]([C@H]2CO)O)[C@H]([C@@H](O2)O[C@@]([C@H](O)CO)([H])[C@]([C@H](C=O)NC(C)=O)([H])O[C@H](O[C@H]3C)[C@H]([C@@H]([C@@H]3O)O)O)O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (152.30 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2184 mL 6.0921 mL 12.1841 mL
5 mM 0.2437 mL 1.2184 mL 2.4368 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.56%

SDS (393 KB)

COA (257 KB) HNMR (290 KB) RP-HPLC (204 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2184 mL 6.0921 mL 12.1841 mL 30.4603 mL
5 mM 0.2437 mL 1.2184 mL 2.4368 mL 6.0921 mL
10 mM 0.1218 mL 0.6092 mL 1.2184 mL 3.0460 mL
15 mM 0.0812 mL 0.4061 mL 0.8123 mL 2.0307 mL
20 mM 0.0609 mL 0.3046 mL 0.6092 mL 1.5230 mL
25 mM 0.0487 mL 0.2437 mL 0.4874 mL 1.2184 mL
30 mM 0.0406 mL 0.2031 mL 0.4061 mL 1.0153 mL
40 mM 0.0305 mL 0.1523 mL 0.3046 mL 0.7615 mL
50 mM 0.0244 mL 0.1218 mL 0.2437 mL 0.6092 mL
60 mM 0.0203 mL 0.1015 mL 0.2031 mL 0.5077 mL
80 mM 0.0152 mL 0.0762 mL 0.1523 mL 0.3808 mL
100 mM 0.0122 mL 0.0609 mL 0.1218 mL 0.3046 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sialyl-Lewis X Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sialyl-Lewis X98603-84-0sLeXEndogenous Metabolitecarcinoma,COLO-205LEC11cellsHL-60CHO-K1PMNC. perfringens sialidaseELAM-1L-selectinP-selectinE-selectinInhibitorinhibitorinhibit

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