2. Epigenetics TGF-beta/Smad
  3. PKC
  4. Spisulosine

Spisulosine (ES-285) is an antiproliferative (antitumoral) compound of marine origin. Spisulosine inhibits the growth of the prostate PC-3 and LNCaP cells through intracellular ceramide accumulation and PKCζ activation.

For research use only. We do not sell to patients.

Spisulosine Chemical Structure

Spisulosine Chemical Structure

CAS No. : 196497-48-0

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Based on 1 publication(s) in Google Scholar

Other Forms of Spisulosine:

Spisulosine Related Antibodies

Top Publications Citing Use of Products

View All PKC Isoform Specific Products:

View All Isoforms
PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Spisulosine (ES-285) is an antiproliferative (antitumoral) compound of marine origin. Spisulosine inhibits the growth of the prostate PC-3 and LNCaP cells through intracellular ceramide accumulation and PKCζ activation[1].

IC50 & Target[1]

PKCζ

 

Cellular Effect
Cell Line Type Value Description References
HBL-100 GI50
2.3 μM
Compound: 1
Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay
[PMID: 25899335]
HeLa GI50
2.6 μM
Compound: 1
Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay
[PMID: 25899335]
LNCaP IC50
1 μM
Compound: 3; ES-285
Cytotoxicity against human LNCaP cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human LNCaP cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 31926468]
PC-3 IC50
1 μM
Compound: 3; ES-285
Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 31926468]
SW1573 GI50
1.3 μM
Compound: 1
Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay
[PMID: 25899335]
T47D GI50
0.9 μM
Compound: 1
Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay
[PMID: 25899335]
WiDr GI50
0.7 μM
Compound: 1
Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay
[PMID: 25899335]
Molecular Weight

285.51

Formula

C18H39NO
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H](N)[C@H](O)CCCCCCCCCCCCCCC
Structure Classification
Initial Source

Spisula polynyma
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 2 mg/mL (7.01 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5025 mL 17.5125 mL 35.0250 mL
5 mM 0.7005 mL 3.5025 mL 7.0050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.00%

SDS (393 KB)

COA (239 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5025 mL 17.5125 mL 35.0250 mL 87.5626 mL
5 mM 0.7005 mL 3.5025 mL 7.0050 mL 17.5125 mL
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Spisulosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spisulosine196497-48-0ES-285ES285ES 285PKCProtein kinase Cceramide accumulationantitumoralPKCζInhibitorinhibitorinhibit

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