Subtilisin  (Synonyms: EC 3.4.21.14)

Subtilisin is a bacterial serine protease. Subtilisin induces Apoptosis. Subtilisin stimulates the expression of pro-allergic cytokines (IL-1α, IL-33). Subtilisin induces prototypic allergic lung inflammation. Subtilisin exhibits anticancer activity against breast and colon cancer. Subtilisin shows antifouling activity. Subtilisin can be used as a detergent additive^{[1][2][3][4][5][6]}.

Product Information
It is a proteolytic enzyme made by deep fermentation, extraction and purification of Bacillus subtilis selected by protoplast mutagenesis method. It can hydrolyze the peptide chain of protein molecules to generate polypeptides or amino acids. It can also catalyze the synthesis of polypeptides in organic solvents. This product contains stabilizers.

Effective temperature range: 20-60℃, the optimum temperature is between 35-45℃.
Effective pH range: 6.0-11.0, the optimum pH range is between 9.5-10.5.
Activators: Ca²⁺, Mg²⁺, Mn²⁺, Zn²⁺, Fe²⁺.
Inhibitors: Reagents acting on serine such as sulfonyl halides.

Instructions
Soluble in 100 mg/mL ddH₂O, avoid repeated freezing and thawing, or dissolve according to specific experimental references.

Subtilisin (3-5 μg/ml; 24 h) shows cytotoxic effects in MCF-7 and HT-29 cancer cells, with MCF-7 cells showing apoptotic cell characteristics and HT-29 cells showing necrotic cell characteristics^[1].
Subtilisin shows antifouling activity^[2].
Subtilisin (1.0-10 μg/ml; 3-6 h) stimulates the expression of pro-allergic cytokines IL-1α, IL-33, TSLP, and the growth factor amphiregulin in H292 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Subtilisin (1 μg; s.c. or i.n.; twice for sensitization and twice for challenge; whole experimental cycle of 22 days) induces prototypic allergic lung inflammation in C57BL/6 mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9014-01-1

Solid

Light yellow to light brown

[Subtilisin]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Singh N, et al. Subtilisin from Bacillus amyloliquefaciens induces apoptosis in breast cancer cells through ubiquitin-proteasome-mediated tubulin degradation. Int J Biol Macromol. 2022 Nov 1;220:852-865.  [Content Brief]

  [2]. Leroy C, et al. Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers. J Appl Microbiol. 2008 Sep;105(3):791-9.  [Content Brief]

  [3]. Alexander PA, et al. Stabilizing mutations and calcium-dependent stability of subtilisin. Biochemistry. 2001 Sep 4;40(35):10640-4.  [Content Brief]

  [4]. Florsheim E, et al. Integrated innate mechanisms involved in airway allergic inflammation to the serine protease subtilisin. J Immunol. 2015 May 15;194(10):4621-30.  [Content Brief]

  [5]. Azrin NAM, et al. Versatility of subtilisin: A review on structure, characteristics, and applications. Biotechnol Appl Biochem. 2022 Dec;69(6):2599-2616.  [Content Brief]

  [6]. Philipp M, et al. Kinetics of subtilisin and thiolsubtilisin. Mol Cell Biochem. 1983;51(1):5-32.  [Content Brief]