2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Subtilisin

Subtilisin, or rubinase, is a bacterial serine protease. Subtilisin can be used as a detergent additive with thermal stability, pH tolerance, and calcium dependent stability.

For research use only. We do not sell to patients.

Subtilisin Chemical Structure

Subtilisin Chemical Structure

CAS No. : 9014-01-1

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Subtilisin Related Antibodies

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Description

Subtilisin, or rubinase, is a bacterial serine protease. Subtilisin can be used as a detergent additive with thermal stability, pH tolerance, and calcium dependent stability[1][2].

In Vitro

Product Information
It is a proteolytic enzyme made by deep fermentation, extraction and purification of Bacillus subtilis selected by protoplast mutagenesis method. It can hydrolyze the peptide chain of protein molecules to generate polypeptides or amino acids. It can also catalyze the synthesis of polypeptides in organic solvents. This product contains stabilizers.

Effective temperature range: 20 - 60℃, the optimum temperature is between 35-45℃.
Effective pH range: 6.0 - 11.0, the optimum PH range is between 9.5-10.5.
Activators: Ca2+, Mg2+, Mn2+, Zn2+, Fe2+.
Inhibitors: Reagents acting on serine such as sulfonyl halides.

Instructions
Soluble in 100 mg/mL ddH2O, avoid repeated freezing and thawing.Or dissolve according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Subtilisin Related Antibodies
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

[Subtilisin]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
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Subtilisin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Subtilisin9014-01-1EC 3.4.21.14Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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