Synucleozid (Synonyms: NSC 377363)

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Synucleozid (NSC 377363) is a potent inhibitor of the SNCA mRNA that encodes α-synuclein protein. Synucleozid selectively targets the α-synuclein mRNA 5′ UTR at the designed IRE site, decreases the amount of SNCA mRNA loaded into polysomes and thereby inhibits SNCA translation. Synucleozid has the potential for the investigation of Parkinson’s disease.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Synucleozid hydrochloride) that retains the same biological activity.

For research use only. We do not sell to patients.

Synucleozid Chemical Structure

CAS No. : 502139-01-7

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Other Forms of Synucleozid:

Synucleozid hydrochloride Get quote

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CMK	IC₅₀	1.31 μM Compound: K313	Antiproliferative activity against human CMK cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human CMK cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay	[PMID: 34046601]
HEL	IC₅₀	2.27 μM Compound: K313	Antiproliferative activity against human HEL cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human HEL cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay	[PMID: 34046601]
K562	IC₅₀	1.08 μM Compound: K313	Antiproliferative activity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay	[PMID: 34046601]
MEG-01	IC₅₀	1.03 μM Compound: K313	Antiproliferative activity against human MEG-01 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human MEG-01 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo assay	[PMID: 34046601]

In Vitro

Synucleozid (0.25-1 μM; 24 hours) abrogates cytotoxicity induced by α-synuclein preformed fibrils, which act as seeds and recruit endogenous α-synuclein to aggregate^[1].
Synucleozid (0-1 μM; 24 hours) binds to the A bulge near the base of the IRE hairpin, reduced levels of α-synuclein in a dose-dependent manner with an IC₅₀ of 500 nM, and inhibits α-synuclein protein expression in SH-SY5Y neuroblastoma cells^[1].
Synucleozid (100 nM-100 μM; 24 hours) binds to 2-AP-labeled and native IRE RNA with similar affinities. It decreases 2-AP emission with an EC₅₀ value of 2.7 ± 0.4 μM, recovery of 2-AP emissions is observed as a function of unlabeled SNCA IRE RNA (RNA-0) concentration, affording a competitive K_d of 1.5 ± 0.3 μM^[1].
Synucleozid (0.25-1 μM; 24 hours) decreases α-synuclein and other proteins that have IREs in their mRNA’s UTR including APP, PrP, Ferritin and TfR as a dose-dependent mannner. All panels is completed in SH-SY5Y cells, except for PrP protein which is assessed in Neuro-2A cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SH-SY5Y neuroblastoma cells
Concentration:	0.25 μM, 0.5 μM, 1 μM
Incubation Time:	24 hours
Result:	Decreased LDH release as a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	SH-SY5Y neuroblastoma cells
Concentration:	0.25 μM; 0.5 μM; 1 μM;
Incubation Time:	24 hours
Result:	Decreased α-synuclein expression as a concentration-dependent manner.

Molecular Weight

368.43

Formula

C₂₂H₂₀N₆

CAS No.

502139-01-7

SMILES

N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(NC4=CC=C(C(N)=N)C=C4)C=C3)N2)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhang P, et al. Translation of the intrinsically disordered protein α-synuclein is inhibited by a small molecule targeting its structured mRNA.Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1457-1467. [Content Brief]

Synucleozid Related Classifications

Neurological Disease

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Synucleozid502139-01-7NSC 377363NSC377363NSC-377363DNA/RNA Synthesisiron-responsiveelementIREParkinsondiseasePDtranslationα-synucleinInhibitorinhibitorinhibit

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