Tetrazine-SS-Biotin

Name: Tetrazine-SS-Biotin
Brand: MedChemExpress
SKU: HY-136031
Rating: 4.5 (1 reviews)

Cat. No.: HY-136031 Purity: 96.12%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetrazine-SS-Biotin is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Tetrazine-SS-Biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-SS-Biotin Chemical Structure

CAS No. : 2123482-78-8

Size	Stock	Quantity
Free Sample (0.1 - 0.5 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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•Related Small Molecules:

•Related Proteins:

View All ADC Linker Isoform Specific Products:

View All Isoforms

Cleavable Linker Non-cleavable Linker Acid Cleavable Linker Disulfide Cleavable Linker Protease Cleavable Linker Glycosidase Cleavable Linker Phosphatase Cleavable Linker

Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetrazine-SS-Biotin is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. Tetrazine-SS-Biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Disulfide Cleavable Linker

Cleavable Linker

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

576.76

Formula

C₂₄H₃₂N₈O₃S₃

CAS No.

2123482-78-8

Appearance

Solid

Color

Pale purple to light pink

SMILES

O=C(NCCSSCCC(NCC1=CC=C(C2=NN=CN=N2)C=C1)=O)CCCC[C@@H]3SC[C@]([C@]3([H])N4)([H])NC4=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (216.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7338 mL	8.6691 mL	17.3382 mL
5 mM	0.3468 mL	1.7338 mL	3.4676 mL
10 mM	0.1734 mL	0.8669 mL	1.7338 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 96.12%

Select Batch:

Data Sheet (267 KB) SDS (393 KB)

COA (244 KB) HNMR (280 KB) RP-HPLC (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7338 mL	8.6691 mL	17.3382 mL	43.3456 mL
	5 mM	0.3468 mL	1.7338 mL	3.4676 mL	8.6691 mL
	10 mM	0.1734 mL	0.8669 mL	1.7338 mL	4.3346 mL
	15 mM	0.1156 mL	0.5779 mL	1.1559 mL	2.8897 mL
	20 mM	0.0867 mL	0.4335 mL	0.8669 mL	2.1673 mL
	25 mM	0.0694 mL	0.3468 mL	0.6935 mL	1.7338 mL
	30 mM	0.0578 mL	0.2890 mL	0.5779 mL	1.4449 mL
	40 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0836 mL
	50 mM	0.0347 mL	0.1734 mL	0.3468 mL	0.8669 mL
	60 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7224 mL
	80 mM	0.0217 mL	0.1084 mL	0.2167 mL	0.5418 mL
	100 mM	0.0173 mL	0.0867 mL	0.1734 mL	0.4335 mL