Tetrazine-SS-NHS

Cat. No.: HY-136032 Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetrazine-SS-NHS is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Tetrazine-SS-NHS is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-SS-NHS Chemical Structure

Size		Stock
5 mg		Check price and availability
10 mg		Check price and availability

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetrazine-SS-NHS is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. Tetrazine-SS-NHS is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Disulfide Cleavable Linker

Cleavable Linker

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

476.53

Formula

C₁₉H₂₀N₆O₅S₂

Appearance

Solid

Color

Orange to red

SMILES

O=C(CCSSCCC(ON1C(CCC1=O)=O)=O)NCC(C=C2)=CC=C2C3=NN=CN=N3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (524.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0985 mL	10.4925 mL	20.9850 mL
5 mM	0.4197 mL	2.0985 mL	4.1970 mL
10 mM	0.2099 mL	1.0493 mL	2.0985 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (268 KB) SDS (251 KB)

COA (245 KB) HNMR (276 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0985 mL	10.4925 mL	20.9850 mL	52.4626 mL
	5 mM	0.4197 mL	2.0985 mL	4.1970 mL	10.4925 mL
	10 mM	0.2099 mL	1.0493 mL	2.0985 mL	5.2463 mL
	15 mM	0.1399 mL	0.6995 mL	1.3990 mL	3.4975 mL
	20 mM	0.1049 mL	0.5246 mL	1.0493 mL	2.6231 mL
	25 mM	0.0839 mL	0.4197 mL	0.8394 mL	2.0985 mL
	30 mM	0.0700 mL	0.3498 mL	0.6995 mL	1.7488 mL
	40 mM	0.0525 mL	0.2623 mL	0.5246 mL	1.3116 mL
	50 mM	0.0420 mL	0.2099 mL	0.4197 mL	1.0493 mL
	60 mM	0.0350 mL	0.1749 mL	0.3498 mL	0.8744 mL
	80 mM	0.0262 mL	0.1312 mL	0.2623 mL	0.6558 mL
	100 mM	0.0210 mL	0.1049 mL	0.2099 mL	0.5246 mL

Tetrazine-SS-NHS Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrazine-SS-NHSADC LinkerAntibody-drug conjugates linkerInhibitorinhibitorinhibit

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