TMB dihydrochloride x.hydrate  (Synonyms: Sure Blue TMB dihydrochloride x.hydrate)

TMB (dihydrochloride x.hydrate) is the dihydrochloride x hydrate form of TMB (HY-15930). TMB (dihydrochloride x.hydrate) is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB (dihydrochloride x.hydrate) can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB (dihydrochloride x.hydrate) can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922^[1][2].

TMB (dihydrochloride x.hydrate) can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C₁₆H₂₂Cl₂N₂.xH₂O

207738-08-7

Solid

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[x].Cl.Cl.O

Room temperature in continental US; may vary elsewhere.

Store at room temperature

• [1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952.  [Content Brief]

  [2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.  [Content Brief]