2. Biochemical Assay Reagents
  3. Enzyme Substrates

Enzyme Substrates

Enzyme Substrates (197):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-126388
    Trypsin Inhibitor, soybean 9035-81-8
    Trypsin Inhibitor, soybean is a potent and reversible inhibitor of trypsin.
    Trypsin Inhibitor, soybean
  • HY-116285
    n-Octyl β-D-glucopyranoside 29836-26-8 ≥98.0%
    n-Octyl-β-d-glucopyranoside is a non-ionic detergent, it can be widely used in the research of biotechnical, biochemical applications, solubilization and crystallization of membrane proteins. n-Octyl-β-d-glucopyranoside can completely inhibit cavitation-induced cell lysis in vitro.
    n-Octyl β-D-glucopyranoside
  • HY-D0714
    Tetrazolium Red 298-96-4 99.85%
    Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride; TTC) is a not brain-penetrant, colorless, water-soluble dye that is reduced by mitochondrial enzymes to a deep red, water-insoluble compound (formazan) mainly in the mitochondria of living cells. Tetrazolium Red is used to observe the activity of dehydrogenase, and it turns colorless to red when exposed to hydrogen. Tetrazolium Red distinguishes between surviving and infarcted brain tissue after stroke. Tetrazolium Red has been used to stain heart tissue to measure the extent of acute lesions and also used to stain brain tissue to detect the size of the infarcted area. The absorption wavelength of Tetrazolium Red is 570 nm.
    Tetrazolium Red
  • HY-15927
    4-Nitrophenyl β-D-glucopyranoside 2492-87-7 99.69%
    4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside can be used to measure of β-glucosidase activity.
    4-Nitrophenyl β-D-glucopyranoside
  • HY-15926
    ONPG 369-07-3 99.84%
    ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
    ONPG
  • HY-W011704
    Phosphoenolpyruvic acid cyclohexylammoniu​m salt 10526-80-4 ≥98.0%
    Phosphoenolpyruvic acid cyclohexylammonium salt is the cyclohexylammonium salt form of phosphoenolpyruvic acid. Phosphoenolpyruvic acid cyclohexylammonium salt is involved in glycolysis and gluconeogenesis, and used as energy source to produce ATP, under the energy-limited conditions. Phosphoenolpyruvic acid cyclohexylammonium salt also exhibits cytoprotective and anti-oxidative properties.
    Phosphoenolpyruvic acid cyclohexylammoniu​m salt
  • HY-137841
    L-Arginine-7-amido-4-methylcoumarin hydrochloride 69304-16-1 99.23%
    L-Arginine-7-amido-4-methylcoumarin (Arginine 4-methyl-7-coumarylamide) hydrochloride is a specific substrate of cathepsin H but not for cathepsins L and B.
    L-Arginine-7-amido-4-methylcoumarin hydrochloride
  • HY-W100403
    Choline tosylate 55357-38-5 99.41%
    Choline tosylate is a nucleophilic compound that inhibits phospholipase A2 and phospholipase C. Choline tosylate inhibits tumor growth in mice by inhibiting the formation of diacylglycerol (DAG).
    Choline tosylate
  • HY-D0719
    Fluorescein Diacetate 596-09-8 99.87%
    Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
    Fluorescein Diacetate
  • HY-D0183
    ATP-polyamine-biotin 1800401-93-7 98.06%
    ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.
    ATP-polyamine-biotin
  • HY-B0445A
    NAD sodium 20111-18-6 99.07%
    NAD (β-Nicotinamide Adenine Dinucleotide) sodium is an analogue of NAD. NAD sodium can be reduced to β-nicotinamide adenine dinucleotide (NADH) during coupling with reactions which oxidize organic substrates. NAD sodium can be converted to β-nicotinamide adenine dinucleotide (NADH) and passes to the inside of mitochondria that indirectly generates ATP.
    NAD sodium
  • HY-W014449
    4-Nitrophenyl butyrate 2635-84-9 99.66%
    4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.
    4-Nitrophenyl butyrate
  • HY-W016412
    Coenzyme Q0 605-94-7 99.88%
    Coenzyme Q0 (CoQ0) is a potent, oral active ubiquinone compound can be derived from Antrodia cinnamomea. Coenzyme Q0 induces apoptosis and autophagy, suppresses of HER-2/AKT/mTOR signaling to potentiate the apoptosis and autophagy mechanisms. Coenzyme Q0 regulates NFκB/AP-1 activation and enhances Nrf2 stabilization in attenuation of inflammation and redox imbalance. Coenzyme Q0 has anti-angiogenic activity through downregulation of MMP-9/NF-κB and upregulation of HO-1 signaling.
    Coenzyme Q0
  • HY-116022A
    4-Nitrophenyl phosphate disodium hexahydrate 333338-18-4 99.80%
    4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
    4-Nitrophenyl phosphate disodium hexahydrate
  • HY-W011012
    Adenosine 5'-monophosphate disodium 4578-31-8 99.63%
    Adenosine 5'-monophosphate disodium is an orally active purine nucleotide, and participates in ATP metabolism. Adenosine 5'-monophosphate disodium is also a ligand for adenosine 2B receptor. Adenosine 5'-monophosphate disodium can activate AMPK in skeletal muscle, and ameliorates insulin resistance and impaired glucose metabolism. Adenosine 5'-monophosphate disodium can be used for research of diabetes.
    Adenosine 5'-monophosphate disodium
  • HY-W017386
    3-Methyl-2-oxovaleric acid sodium 3715-31-9 99.60%
    3-Methyl-2-oxovaleric acid, sodium salt belongs to the class of carboxylic acids, consisting of a five-carbon chain with methyl, carboxyl and ketone groups. This compound is commonly used as an analytical reagent in biochemical and medical research, especially for the detection and quantification of 2-ketoacids. It can also be used as a substrate in enzyme assays to measure the activity of certain enzymes involved in amino acid metabolism. In addition, 3-Methyl-2-oxovaleric acid, sodium salt may have potential research roles in various diseases such as diabetes, cancer and cardiovascular disease.
    3-Methyl-2-oxovaleric acid sodium
  • HY-134426
    DL-β-Hydroxybutyryl coenzyme A lithium 103404-51-9 ≥98.0%
    DL-β-Hydroxybutyryl coenzyme A lithium is an intermediate in the fermentation of butyric acid and the metabolism of lysine and tryptophan, and is produced from β-hydroxybutyric acid by short-chain-CoA synthase.
    DL-β-Hydroxybutyryl coenzyme A lithium
  • HY-D0904
    Acetylthiocholine iodide 1866-15-5 99.87%
    Acetylthiocholine iodide can be used as a substrate for certain enzymes, such as cholinesterase, etc., and can be used to determine the activity level of these enzymes. In addition, the compound is used in some medical research, for example in the fields of neuroscience and organ physiology.
    Acetylthiocholine iodide
  • HY-15906
    AMPPD 122341-56-4 99.93%
    AMPPD, a 1,2-dioxo-cyclohexane derivative, is a biochemistry ultrasensitive alkaline phosphatase substrate.
    AMPPD
  • HY-137855
    4-Methylumbelliferyl sulfate potassium 15220-11-8 99.29%
    4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
    4-Methylumbelliferyl sulfate potassium