2. Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Fluorescent Dye Reactive Oxygen Species
  4. TMB monosulfate

TMB monosulfate  (Synonyms: BM blue monosulfate; Sure Blue TMB monosulfate)

Cat. No.: HY-15930C Purity: 99.99%
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TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

TMB monosulfate Chemical Structure

TMB monosulfate Chemical Structure

CAS No. : 54827-18-8

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Based on 1 publication(s) in Google Scholar

Other Forms of TMB monosulfate:

TMB monosulfate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TMB monosulfate

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  • Purity & Documentation

  • References

  • Customer Review

Description

TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
In Vitro

TMB monosulfate can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TMB monosulfate Related Antibodies
Molecular Weight

338.42

Formula

C16H22N2O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.O=S(O)(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 28 mg/mL (82.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 2.78 mg/mL (8.21 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9549 mL 14.7745 mL 29.5491 mL
5 mM 0.5910 mL 2.9549 mL 5.9098 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.9549 mL 14.7745 mL 29.5491 mL 73.8727 mL
5 mM 0.5910 mL 2.9549 mL 5.9098 mL 14.7745 mL
DMSO 10 mM 0.2955 mL 1.4775 mL 2.9549 mL 7.3873 mL
15 mM 0.1970 mL 0.9850 mL 1.9699 mL 4.9248 mL
20 mM 0.1477 mL 0.7387 mL 1.4775 mL 3.6936 mL
25 mM 0.1182 mL 0.5910 mL 1.1820 mL 2.9549 mL
30 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4624 mL
40 mM 0.0739 mL 0.3694 mL 0.7387 mL 1.8468 mL
50 mM 0.0591 mL 0.2955 mL 0.5910 mL 1.4775 mL
60 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
80 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9234 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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TMB monosulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TMB monosulfate54827-18-8BM blue monosulfate Sure Blue TMB monosulfateFluorescent DyeReactive Oxygen Speciesdyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit

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