TPC2-A1-P

Name: TPC2-A1-P
Brand: MedChemExpress
SKU: HY-131615
Rating: 5.0 (2 reviews)

Cat. No.: HY-131615 Purity: 99.23%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TPC2-A1-P is a powerful and membrane permeable agonist of two pore channel 2 (TPC2) with an EC₅₀ of 10.5 μM. TPC2-A1-P plays its role by mimicking the physiological actions of PI(3,5)P2. TPC2-A1-P also shows higher potency to induce Na²⁺ mobilisation from TPC2 than TPC-A1-N (HY-131614). TPC2-A1-P can be used to probe different functions of TPC2 channels in intact cells.

For research use only. We do not sell to patients.

TPC2-A1-P Chemical Structure

CAS No. : 2804595-86-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 304	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 304	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2350	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE TPC2-A1-P

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View All Isoforms

Na_v1.1 Na_v1.2 Na_v1.3 Na_v1.4 Na_v1.5 Na_v1.6 Na_v1.7 Na_v1.8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EC50: 10.5 μM (TPC2)^[2]

In Vitro

Two-pore channels (TPC1-3) are ancient members of the voltage-gated ion channel superfamily. TPCs are expressed throughout the endo-lysosomal system and regulates the trafficking of various cargoes^[1].
TPC2 can mediate different physiological and possibly pathophysiological effects depending on how it is activated.?The?ion selectivity of TPC2 is not fixed but rather agonist-dependent. TPC2 is a unique example of an ion channel that conducts different ions in response to different activating ligands^[1].
TPC2-A1-P (10 μM) reproducibly evokes Ca²⁺?signals, and TPC2-A1-P response reachs its plateau slower than TPC2-A1-N (HY-131614). The EC₅₀?in full concentration-effect relationships for the plateau response is 10.5 μM for TPC2-A1-P in a cell line stably expressing TPC2^L11A/L12A.
TPC2-A1-P (10-30 μM) induces Ca²⁺?signals in Hela cells expressing TPC2 in the presence but not absence of extracellular Ca²⁺. However, the responses are smaller and delayed compared to TPC2-A1-N (HY-131614), consistent with the results obtained in cells stably expressing TPC2^L11A/L12A. TPC2-A1-P fails to induce Ca²⁺?signals in cells expressing ‘pore-dead’ TPC2^{L11A/L12A/L265P} and also fails to evoke Ca²⁺?signals in cells expressing human TRPML1 re-routed to the plasma membrane (TRPML1^ΔNC)^[1].
In endo-lysosomal patch-clamp experiments, TPC2-A1-P (10 μM) evokes currents in endo-lysosomes isolated from cells expressing TPC2 and TPC2^M484L, the currents evoked by TPC2-A1-P are significantly larger than those evoked by TPC2-A1-N (HY-131614) in both wild-type and gain-of-function variant,and exhibits an EC₅₀?value of 0.6 μM for TPC2-A1-P^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

460.28

Formula

C₂₀H₂₁BrF₃NO₃

CAS No.

2804595-86-4

Appearance

Solid

Color

White to light yellow

SMILES

BrC1=CC(C2=CC(C(O)=O)=C(C)N2CC3CCCCC3)=C(OC(F)(F)F)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (108.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1726 mL	10.8630 mL	21.7259 mL
5 mM	0.4345 mL	2.1726 mL	4.3452 mL
10 mM	0.2173 mL	1.0863 mL	2.1726 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (257 KB) HNMR (227 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Susanne Gerndt, et al. Agonist-mediated switching of ion selectivity in TPC2 differentially promotes lysosomal function. Elife. 2020 Mar 16;9:e54712. [Content Brief]

[2]. Gerndt S, et al. Discovery of lipophilic two-pore channel agonists. FEBS J. 2020;287(24):5284-5293. [Content Brief]

[3]. Xuhui Jin, et al. Targeting Two-Pore Channels: Current Progress and Future Challenges. Trends Pharmacol Sci. 2020 Aug;41(8):582-594. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1726 mL	10.8630 mL	21.7259 mL	54.3148 mL
	5 mM	0.4345 mL	2.1726 mL	4.3452 mL	10.8630 mL
	10 mM	0.2173 mL	1.0863 mL	2.1726 mL	5.4315 mL
	15 mM	0.1448 mL	0.7242 mL	1.4484 mL	3.6210 mL
	20 mM	0.1086 mL	0.5431 mL	1.0863 mL	2.7157 mL
	25 mM	0.0869 mL	0.4345 mL	0.8690 mL	2.1726 mL
	30 mM	0.0724 mL	0.3621 mL	0.7242 mL	1.8105 mL
	40 mM	0.0543 mL	0.2716 mL	0.5431 mL	1.3579 mL
	50 mM	0.0435 mL	0.2173 mL	0.4345 mL	1.0863 mL
	60 mM	0.0362 mL	0.1810 mL	0.3621 mL	0.9052 mL
	80 mM	0.0272 mL	0.1358 mL	0.2716 mL	0.6789 mL
	100 mM	0.0217 mL	0.1086 mL	0.2173 mL	0.5431 mL

TPC2-A1-P Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPC2-A1-P2804595-86-4Sodium ChannelNa channelsNa+ channelsNAADPnicotinic acidadenine dinucleotide phosphatelysosomalPI(3,5)P2TPC2Two-pore channelInhibitorinhibitorinhibit

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TPC2-A1-P

Customer Review

TPC2-A1-P Related Antibodies

2 Publications Citing Use of MCE TPC2-A1-P

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sodium Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

TPC2-A1-P Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

TPC2-A1-P Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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