1. Others
  2. Biochemical Assay Reagents
  3. TPCK-treated Trypsin

TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research.

For research use only. We do not sell to patients.

TPCK-treated Trypsin Chemical Structure

TPCK-treated Trypsin Chemical Structure

CAS No. : 9002-07-7

Size Price Stock Quantity
5 mg USD 50 In-stock
10 mg USD 75 In-stock
25 mg USD 145 In-stock
50 mg USD 240 In-stock
100 mg USD 380 In-stock
200 mg   Get quote  
500 mg   Get quote  

Get it tomorrow March 27 by noon. Order within 7 hrs 57 mins.

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of TPCK-treated Trypsin:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research[1].

In Vitro

Product Information
This product is derived from bovine pancreas. After chromatographic purification, it is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit the activity of proteases. The theoretical molecular weight is 23.3 kDa, and the optimal pH is 7.5-8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.

Instructions (for reference only)
This product can be directly used for protein enzymatic cleavage. The recommended enzymatic cleavage ratio is 1:50-1:100 (w/w);
Enzymatic cleavage overnight at 37℃.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[TPCK-treated Trypsin]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

TPCK-treated Trypsin Related Classifications

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
TPCK-treated Trypsin
Cat. No.:
HY-129047C
Quantity:
MCE Japan Authorized Agent: