TPCK-treated Trypsin

Cat. No.: HY-129047C: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research.

For research use only. We do not sell to patients.

TPCK-treated Trypsin Chemical Structure

CAS No. : 9002-07-7

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of TPCK-treated Trypsin:

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description	TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research^[1].
In Vitro	Product Information This product is derived from bovine pancreas. After chromatographic purification, it is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit the activity of proteases. The theoretical molecular weight is 23.3 kDa, and the optimal pH is 7.5-8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis. Instructions (for reference only) This product can be directly used for protein enzymatic cleavage. The recommended enzymatic cleavage ratio is 1:50-1:100 (w/w); Enzymatic cleavage overnight at 37℃. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. TPCK-treated Trypsin Related Antibodies
CAS No.	9002-07-7
Appearance	Solid
Color	White to off-white
SMILES	[TPCK-treated Trypsin]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility	In Vitro: H₂O : 100 mg/mL (Need ultrasonic) Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: sales@medchemexpress.com Technical Support: tech@medchemexpress.com
Purity & Documentation	Select Batch: Data Sheet (264 KB) SDS (480 KB) COA (242 KB) Handling Instructions (2659 KB)
References	[1]. Shuford CM, et al., Cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification. J Mass Spectrom Adv Clin Lab. 2023 Nov 23;30:74-82. [Content Brief]