2. Metabolic Enzyme/Protease GPCR/G Protein
  3. Ser/Thr Protease Protease Activated Receptor (PAR)
  4. Trypsin

Trypsin is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation.

For research use only. We do not sell to patients.

Trypsin Chemical Structure

Trypsin Chemical Structure

CAS No. : 9002-07-7

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Based on 5 publication(s) in Google Scholar

Other Forms of Trypsin:

Trypsin Related Antibodies

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Description

Trypsin is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

IC50 & Target

PAR2

 

PAR4

 

In Vitro

Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2].
Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2].
Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3].
Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Trypsin Related Antibodies

Western Blot Analysis[2]

Cell Line: LLC-PK cells, ST cells
Concentration: 5 μg/mL
Incubation Time: 24 or 48 h
Result: Promoted PDCoV replication in LLC-PK cells but not ST cells.

Immunofluorescence[2]

Cell Line: LLC-PK cells, ST cells
Concentration: 10 and 50 ng/mL
Incubation Time: 12 h
Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.
In Vivo

Trypsin (100-500?μg per site in 50?μL saline, intradermal injection) induces scratching behaviour in mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss mice[5]
Dosage: 100-500 μg per site, in saline (50 μL)
Administration: Intradermal injection
Result: Induced pruritus, and was inhibited by trypsin inhibitor.
Clinical Trial
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

[Trypsin]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (480 KB)

COA (231 KB)

Handling Instructions (2659 KB)
References
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Trypsin Related Classifications

  • Molarity Calculator

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trypsin9002-07-7Ser/Thr ProteaseProtease Activated Receptor (PAR)Serine proteasesSerine endopeptidasesThreonine proteasesThrombin receptorsPDCoVvirus releasefusionLLC-PKC6PBMCpruritusinflammationitchingInhibitorinhibitorinhibit

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