Tris(3-hydroxypropyltriazolylmethyl)amine (Synonyms: THPTA)

Cat. No.: HY-W021042 Purity: 98.75%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) is an accelerating ligand in the copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) and protects cells from oxidants generated by copper-catalyzed reduction of oxygen by ascorbate. In addition, Tris(3-hydroxypropyltriazolylmethyl)amine can also protect the histidine moiety of biomolecules in a manner proportional to the ligand concentration.

For research use only. We do not sell to patients.

Tris(3-hydroxypropyltriazolylmethyl)amine Chemical Structure

CAS No. : 760952-88-3

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Description

In Vitro

Tris(3-hydroxypropyltriazolylmethyl)amine (1.25 mM, 1 h) intercepts and quickly reduce reactive oxygen species generated by the ascorbate-driven reduction of dissolved O₂ without compromising the CuAAC reaction rate very much^[1].
Tris(3-hydroxypropyltriazolylmethyl)amine (250 μM, 1-5 min) reverses the toxic effect of Cu-ascorbate effect in a dose-dependent manner at a 5:1 ligand:Cu ratio, preserving the viability of HeLa, Chinese hamster ovary (CHO), and Jurkat cells^[2].
Tris(3-hydroxypropyltriazolylmethyl)amine (125 μM, 5 min) accelerates the click reaction and allows the cells to survive at a copper concentration of 50 μM in HeLa cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[4]

Cell Line:	HeLa cells
Concentration:	125 μM
Incubation Time:	5 min
Result:	Resulted the HeLa cells alive up to the copper concentration of 50 μM.

Molecular Weight

434.50

Formula

C₁₈H₃₀N₁₀O₃

CAS No.

760952-88-3

Appearance

Solid

Color

White to light brown

SMILES

OCCCN1N=NC(CN(CC2=CN(CCCO)N=N2)CC3=CN(CCCO)N=N3)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 175 mg/mL (402.76 mM)

DMSO : 100 mg/mL (230.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3015 mL	11.5075 mL	23.0150 mL
5 mM	0.4603 mL	2.3015 mL	4.6030 mL
10 mM	0.2301 mL	1.1507 mL	2.3015 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (250 KB) HNMR (114 KB) LCMS (255 KB)

Handling Instructions (2659 KB)

References

[1]. Hong V, et al. Analysis and optimization of copper-catalyzed azide-alkyne cycloaddition for bioconjugation. Angew Chem Int Ed Engl. 2009;48(52):9879-83. [Content Brief]

[2]. Hong V, et al. Labeling live cells by copper-catalyzed alkyne− azide click chemistry. Bioconjugate chemistry 21.10 (2010): 1912-1916. [Content Brief]

[3]. Rathinam S, et al. Conjugation of CRAMP18-35 Peptide to Chitosan and Hydroxypropyl Chitosan via Copper-Catalyzed Azide-Alkyne Cycloaddition and Investigation of Antibacterial Activity. Int J Mol Sci. 2024 Aug 30;25(17):9440. [Content Brief]

[4]. Yuan Y, et al. Bioorthogonal Turn-On Probe Based on Aggregation-Induced Emission Characteristics for Cancer Cell Imaging and Ablation. Angew Chem Int Ed Engl. 2016 May 23;55(22):6457-61. [Content Brief]

[5]. Kozarski M, et al. Towards superior mRNA caps accessible by click chemistry: synthesis and translational properties of triazole-bearing oligonucleotide cap analogs. RSC Adv. 2023 Apr 25;13(19):12809-12824. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.3015 mL	11.5075 mL	23.0150 mL	57.5374 mL
	5 mM	0.4603 mL	2.3015 mL	4.6030 mL	11.5075 mL
	10 mM	0.2301 mL	1.1507 mL	2.3015 mL	5.7537 mL
	15 mM	0.1534 mL	0.7672 mL	1.5343 mL	3.8358 mL
	20 mM	0.1151 mL	0.5754 mL	1.1507 mL	2.8769 mL
	25 mM	0.0921 mL	0.4603 mL	0.9206 mL	2.3015 mL
	30 mM	0.0767 mL	0.3836 mL	0.7672 mL	1.9179 mL
	40 mM	0.0575 mL	0.2877 mL	0.5754 mL	1.4384 mL
	50 mM	0.0460 mL	0.2301 mL	0.4603 mL	1.1507 mL
	60 mM	0.0384 mL	0.1918 mL	0.3836 mL	0.9590 mL
	80 mM	0.0288 mL	0.1438 mL	0.2877 mL	0.7192 mL
	100 mM	0.0230 mL	0.1151 mL	0.2301 mL	0.5754 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tris(3-hydroxypropyltriazolylmethyl)amine Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tris(3-hydroxypropyltriazolylmethyl)amine760952-88-3THPTABiochemical Assay ReagentsCopper catalysisazide-alkyne cycloaddition reactionclick chemistry reactionInhibitorinhibitorinhibit

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