True Blue  (Synonyms: NCI 240899)

True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival^[1][2].

True Blue can label dorsal root ganglion neurons (DRGNs) and motoneurons (MNs) by soaking the cut sciatic nerve of adult female rats, and does not affect neuronal survival whenever 4 days or 20 weeks after soak^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

417.29

C₂₀H₁₈Cl₂N₄O₂

71431-30-6

Solid

Light yellow to yellow

418

395

N=C(C1=CC=C(OC(/C=C/C2=CC3=CC(C(N)=N)=CC=C3O2)=C4)C4=C1)N.[H]Cl.[H]Cl

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. M Bentivoglio, et al. Fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich DNA. Neurosci Lett. 1979 May;12(2-3):235-40.  [Content Brief]

  [2]. W T Garrett, et al. Fluoro-Gold's toxicity makes it inferior to True Blue for long-term studies of dorsal root ganglion neurons and motoneurons. Neurosci Lett. 1991 Jul 8;128(1):137-9.  [Content Brief]