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Trx-red  (Synonyms: NBL-SS perchlorate)

Cat. No.: HY-D1254 Purity: 96.58%
COA Handling Instructions

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Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (λex=615 nm, λem=661 nm).

For research use only. We do not sell to patients.

Trx-red Chemical Structure

Trx-red Chemical Structure

CAS No. : 2368978-96-3

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5 mg USD 350 In-stock
10 mg USD 580 In-stock
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Based on 1 publication(s) in Google Scholar

Trx-red Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (λex=615 nm, λem=661 nm)[1].

In Vitro

Trx-red (NBL-SS) displayed multiple favorable properties, such as red emission, fast response, and high fluorescence signal. Thioredoxin (Trx) is a ubiquitous redox-regulating protein essentially involved in cell growth, differentiation, and death[1].
When HeLa cells are incubated with Trx-red (NBL-SS; 5 μM; 0-30 min), a clear time-dependent increase of the fluorescence is observed[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Trx-red Related Antibodies
In Vivo

Trx-red (NBL-SS; 10 μM; 30 min) is also suitable to image thioredoxin in 6 day-old zebrafish larvae[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

714.16

Formula

C29H32ClN3O12S2
CAS No.
Appearance

Oil

Color

Dark purple to black

Emission (Em)

660
Excitation (Ex)

615
SMILES

O=C(NC1=CC(OC2=C/3)=C(C4=C1C=CC=C4)N=C2C=CC3=[N+](CCC(OC)=O)/CCC(OC)=O)OCCSSCCO.O=Cl(=O)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (70.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4002 mL 7.0012 mL 14.0025 mL
5 mM 0.2800 mL 1.4002 mL 2.8005 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.58%

COA (247 KB) LCMS (455 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4002 mL 7.0012 mL 14.0025 mL 35.0062 mL
5 mM 0.2800 mL 1.4002 mL 2.8005 mL 7.0012 mL
10 mM 0.1400 mL 0.7001 mL 1.4002 mL 3.5006 mL
15 mM 0.0933 mL 0.4667 mL 0.9335 mL 2.3337 mL
20 mM 0.0700 mL 0.3501 mL 0.7001 mL 1.7503 mL
25 mM 0.0560 mL 0.2800 mL 0.5601 mL 1.4002 mL
30 mM 0.0467 mL 0.2334 mL 0.4667 mL 1.1669 mL
40 mM 0.0350 mL 0.1750 mL 0.3501 mL 0.8752 mL
50 mM 0.0280 mL 0.1400 mL 0.2800 mL 0.7001 mL
60 mM 0.0233 mL 0.1167 mL 0.2334 mL 0.5834 mL
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Trx-red Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trx-red2368978-96-3NBL-SSFluorescent Dyefluorescentprobethioredoxinlivecellsred-emittingInhibitorinhibitorinhibit

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