2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. USP8-IN-3

USP8-IN-3 (Compd U51) is a deubiquitinase USP8 inhibitor with an IC50 value of 4.0 μM. USP8-IN-3 also inhibits the proliferation of GH3 and H1957 cells with GI50s of 37.03 μM and 6.01 μM, respectively.

For research use only. We do not sell to patients.

USP8-IN-3 Chemical Structure

USP8-IN-3 Chemical Structure

CAS No. : 2477651-10-6

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Based on 1 publication(s) in Google Scholar

USP8-IN-3 Related Antibodies

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USP1 USP2 USP7 USP8 USP30 UCHL1

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Description

USP8-IN-3 (Compd U51) is a deubiquitinase USP8 inhibitor with an IC50 value of 4.0 μM. USP8-IN-3 also inhibits the proliferation of GH3 and H1957 cells with GI50s of 37.03 μM and 6.01 μM, respectively[1].

IC50 & Target[1]

USP8

4.0 μM (IC50)

In Vitro

USP8-IN-3 (6.25-100 μM; 15 min) inhibits the cleavage activity of USP8 on diubiquitin[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

USP8-IN-3 Related Antibodies

Western Blot Analysis[1]

Cell Line: /
Concentration: 6.25 μM, 12.5 μM, 25 μM, 50 μM, and 100 μM
Incubation Time: 15 min; added Di-Ub substrate for another 1 h
Result: Decreased the cleaved diubiquitin level.
Molecular Weight

425.43

Formula

C18H18F3N5O2S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

[O-][N+](C1=CC=C(NC(NC2CCN(CC2)C3=CC=CC(C(F)(F)F)=N3)=S)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (235.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3506 mL 11.7528 mL 23.5056 mL
5 mM 0.4701 mL 2.3506 mL 4.7011 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3506 mL 11.7528 mL 23.5056 mL 58.7641 mL
5 mM 0.4701 mL 2.3506 mL 4.7011 mL 11.7528 mL
10 mM 0.2351 mL 1.1753 mL 2.3506 mL 5.8764 mL
15 mM 0.1567 mL 0.7835 mL 1.5670 mL 3.9176 mL
20 mM 0.1175 mL 0.5876 mL 1.1753 mL 2.9382 mL
25 mM 0.0940 mL 0.4701 mL 0.9402 mL 2.3506 mL
30 mM 0.0784 mL 0.3918 mL 0.7835 mL 1.9588 mL
40 mM 0.0588 mL 0.2938 mL 0.5876 mL 1.4691 mL
50 mM 0.0470 mL 0.2351 mL 0.4701 mL 1.1753 mL
60 mM 0.0392 mL 0.1959 mL 0.3918 mL 0.9794 mL
80 mM 0.0294 mL 0.1469 mL 0.2938 mL 0.7346 mL
100 mM 0.0235 mL 0.1175 mL 0.2351 mL 0.5876 mL
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USP8-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP8-IN-32477651-10-6DeubiquitinaseDUBsInhibitorinhibitorinhibit

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