2. Stem Cell/Wnt PROTAC
  3. Wnt PROTACs β-catenin
  4. xStAx-VHLL

xStAx-VHLL is a β-catenin PROTAC degrader that promotes ubiquitination and proteasome degradation of β-catenin. xStAx-VHLL inhibits the Wnt/β-catenin signaling pathway. xStAx-VHLL can inhibit the proliferation of colon cancer cells and has anti-tumor activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

xStAx-VHLL Chemical Structure

xStAx-VHLL Chemical Structure

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Based on 1 publication(s) in Google Scholar

Other Forms of xStAx-VHLL:

xStAx-VHLL Related Antibodies

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Description

xStAx-VHLL is a β-catenin PROTAC degrader that promotes ubiquitination and proteasome degradation of β-catenin. xStAx-VHLL inhibits the Wnt/β-catenin signaling pathway. xStAx-VHLL can inhibit the proliferation of colon cancer cells and has anti-tumor activity[1].

In Vitro

xStAx-VHLL (10-60 μM; 1-6 days) induces β-catenin degradation in a dose-dependent manner in colorectal cancer cells and inhibits cell proliferation[1].
xStAx-VHLL (50-90 μM; 24 h) inhibits Wnt/β-catenin signaling in HEK293T cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

xStAx-VHLL Related Antibodies

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 10, 20, 30, 40, 50 and 60 μM
Incubation Time: 24 h
Result: Reduced the level of β-catenin in a dose-dependent manner.
In Vivo

xStAx-VHLL (30 mg/kg; Intraperitoneal injection; 14 days) has antitumor activity in mouse intestinal tumor model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: APC min/+ mice that harbored a point mutation in the APC gene, resulting in APC deletion and constitutive activation of Wnt signaling (APC min/+ mice develops intestinal tumors around 10 weeks-old) [1]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection (i.p.); 14 days
Result: Dramatically reduced tumor number.
Reduced the active of β-catenin protein levels.
Molecular Weight

3231.89

Formula

C154H244N48O29
Appearance

Solid

Color

White to off-white

Sequence

Ac-Arg-Arg-Trp-Pro-Arg-{S5}-Ile-Leu-Asp-{S5}-His-Val-Arg-Arg-Val-Trp-Arg-{Ahx}-Ala-Leu-Ala-Pro-Tyr-Ile-Pro-NH2
Sequence Shortening

Ac-RRWPR{S5}ILD{S5}HVRRVWR-{Ahx}-ALAPYIP-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (30.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3094 mL 1.5471 mL 3.0942 mL
5 mM 0.0619 mL 0.3094 mL 0.6188 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.3094 mL 1.5471 mL 3.0942 mL 7.7354 mL
5 mM 0.0619 mL 0.3094 mL 0.6188 mL 1.5471 mL
10 mM 0.0309 mL 0.1547 mL 0.3094 mL 0.7735 mL
15 mM 0.0206 mL 0.1031 mL 0.2063 mL 0.5157 mL
20 mM 0.0155 mL 0.0774 mL 0.1547 mL 0.3868 mL
25 mM 0.0124 mL 0.0619 mL 0.1238 mL 0.3094 mL
30 mM 0.0103 mL 0.0516 mL 0.1031 mL 0.2578 mL
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xStAx-VHLL Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

xStAx-VHLLWntPROTACsβ-cateninBeta catenincolorectal cancerHCT116 cellsHEK293T cellsInhibitorinhibitorinhibit

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