2. Apoptosis
  3. Caspase Apoptosis
  4. Z-LEHD-FMK

Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-LEHD-FMK Chemical Structure

Z-LEHD-FMK Chemical Structure

CAS No. : 210345-04-3

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Based on 10 publication(s) in Google Scholar

Other Forms of Z-LEHD-FMK:

Z-LEHD-FMK Related Antibodies

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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  • Purity & Documentation

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  • Customer Review

Description

Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma[1][2][3].

IC50 & Target[1]

Caspase-9

 

In Vitro

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Z-LEHD-FMK Related Antibodies

Apoptosis Analysis[1]

Cell Line: SW480, H460, HCT116 and 293 cells
Concentration: 20 μM
Incubation Time: Pretreated for 30 min
Result: Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis[1]

Cell Line: HCT116, SW480 cells
Concentration: 20 μM
Incubation Time: 2 h
Result: Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
In Vivo

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar albino rats (250-350 g) with SCI[2]
Dosage: 0.8 μmol/kg
Administration: I.v. for 1 or 7 days
Result: Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
Molecular Weight

690.72

Formula

C32H43FN6O10
CAS No.
Appearance

Solid

Color

White to yellow

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (144.78 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4478 mL 7.2388 mL 14.4776 mL
5 mM 0.2896 mL 1.4478 mL 2.8955 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4478 mL 7.2388 mL 14.4776 mL 36.1941 mL
5 mM 0.2896 mL 1.4478 mL 2.8955 mL 7.2388 mL
10 mM 0.1448 mL 0.7239 mL 1.4478 mL 3.6194 mL
15 mM 0.0965 mL 0.4826 mL 0.9652 mL 2.4129 mL
20 mM 0.0724 mL 0.3619 mL 0.7239 mL 1.8097 mL
25 mM 0.0579 mL 0.2896 mL 0.5791 mL 1.4478 mL
30 mM 0.0483 mL 0.2413 mL 0.4826 mL 1.2065 mL
40 mM 0.0362 mL 0.1810 mL 0.3619 mL 0.9049 mL
50 mM 0.0290 mL 0.1448 mL 0.2896 mL 0.7239 mL
60 mM 0.0241 mL 0.1206 mL 0.2413 mL 0.6032 mL
80 mM 0.0181 mL 0.0905 mL 0.1810 mL 0.4524 mL
100 mM 0.0145 mL 0.0724 mL 0.1448 mL 0.3619 mL
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Z-LEHD-FMK Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-LEHD-FMK210345-04-3CaspaseApoptosiscaspase-9lethalreperfusioninjuryapoptosisneuroprotectiveInhibitorinhibitorinhibit

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