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  4. 1-Methyl-7-nitroisatoic anhydride

1-Methyl-7-nitroisatoic anhydride  (Synonyms: 1M7)

Cat. No.: HY-D0913 Purity: ≥98.0%
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1-methyl-7-nitroisatoic anhydride (1M7) is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity, can be used for RNA structure analysis.

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1-Methyl-7-nitroisatoic anhydride Chemical Structure

1-Methyl-7-nitroisatoic anhydride Chemical Structure

CAS No. : 73043-80-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
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10 mM * 1 mL in DMSO USD 55 In-stock
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Based on 2 publication(s) in Google Scholar

1-Methyl-7-nitroisatoic anhydride Related Antibodies

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2 Publications Citing Use of MCE 1-Methyl-7-nitroisatoic anhydride

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Description

1-methyl-7-nitroisatoic anhydride (1M7) is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity, can be used for RNA structure analysis[1].

In Vitro

SHAPE reaction protocol for 1-methyl-7-nitroisatoic anhydride[1]
1. RNA pretreatment: Incubate RNA in a buffer containing a final concentration of 10/9 for 30 minutes to allow the RNA to fully fold and reach equilibrium.
2. Prepare 1-methyl-7-nitroisatoic anhydride modification reaction: Prepare a 1-methyl-7-nitroisatoic anhydride solution, and select the appropriate concentration based on the different RNAs (30 mM for 16S and 23S rRNA, 100 mM for RNase P RNA, and 50 mM for HIV-1 RNA). Dissolve 1-methyl-7-nitroisatoic anhydride in DMSO to prepare a 10-fold concentrated solution.
3. Start the 1M7 modification reaction: Add 1/10 volume of 1-methyl-7-nitroisatoic anhydride-DMSO solution to the RNA solution and mix immediately. For the non-reaction control group, only add an equal volume of pure DMSO.
4. Reaction conditions: react at 37°C for a certain time (the specific time depends on the experimental design).
5. Termination of reaction: After the reaction is completed, immediately place the reaction mixture on ice to stop the reaction.
6. RNA recovery: RNA is recovered using ethanol precipitation, the precipitate is collected by centrifugation, and the supernatant is discarded. Resuspend the RNA precipitate with a small amount of 1/23 TE buffer (5 mM Tris-HCl, 0.5 mM EDTA).
7. Analysis of SHAPE reaction products: Use primers with different fluorescent labels to analyze the modification sites of RNA by primer extension. Separate the [+] and [–] reaction products and one or two sequencing gradients by electrophoresis, and read the results by fluorescence detector.
8. Data analysis: Analyze the secondary structure information of RNA based on the changes in the intensity of the fluorescence signal.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1-Methyl-7-nitroisatoic anhydride Related Antibodies
Molecular Weight

222.15

Formula

C9H6N2O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O1)N(C)C2=CC([N+]([O-])=O)=CC=C2C1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (225.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5015 mL 22.5073 mL 45.0146 mL
5 mM 0.9003 mL 4.5015 mL 9.0029 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5015 mL 22.5073 mL 45.0146 mL 112.5366 mL
5 mM 0.9003 mL 4.5015 mL 9.0029 mL 22.5073 mL
10 mM 0.4501 mL 2.2507 mL 4.5015 mL 11.2537 mL
15 mM 0.3001 mL 1.5005 mL 3.0010 mL 7.5024 mL
20 mM 0.2251 mL 1.1254 mL 2.2507 mL 5.6268 mL
25 mM 0.1801 mL 0.9003 mL 1.8006 mL 4.5015 mL
30 mM 0.1500 mL 0.7502 mL 1.5005 mL 3.7512 mL
40 mM 0.1125 mL 0.5627 mL 1.1254 mL 2.8134 mL
50 mM 0.0900 mL 0.4501 mL 0.9003 mL 2.2507 mL
60 mM 0.0750 mL 0.3751 mL 0.7502 mL 1.8756 mL
80 mM 0.0563 mL 0.2813 mL 0.5627 mL 1.4067 mL
100 mM 0.0450 mL 0.2251 mL 0.4501 mL 1.1254 mL
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1-Methyl-7-nitroisatoic anhydride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1-Methyl-7-nitroisatoic anhydride73043-80-81M7Fluorescent DyeInhibitorinhibitorinhibit

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