2. Cell Cycle/DNA Damage
  3. Nucleoside Antimetabolite/Analog
  4. 1-Methylcytosine

1-Methylcytosine is a methylated form of the DNA base cytosine and used as a nucleobase of hachimoji DNA, in which it pairs with Isoguanine.

For research use only. We do not sell to patients.

1-Methylcytosine Chemical Structure

1-Methylcytosine Chemical Structure

CAS No. : 1122-47-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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Based on 1 publication(s) in Google Scholar

1-Methylcytosine Related Antibodies

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  • Purity & Documentation

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Description

1-Methylcytosine is a methylated form of the DNA base cytosine and used as a nucleobase of hachimoji DNA, in which it pairs with Isoguanine[1][2].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
12 μM
Compound: 21
Growth inhibition of human HeLa cells after 72 hrs by MTT assay
Growth inhibition of human HeLa cells after 72 hrs by MTT assay
[PMID: 18640746]
MCF7 IC50
> 100 μM
Compound: 21
Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
Growth inhibition of human MCF7 cells after 72 hrs by MTT assay
[PMID: 18640746]
MIA PaCa-2 IC50
> 100 μM
Compound: 21
Growth inhibition of human MIAPaCa2 cells after 72 hrs by MTT assay
Growth inhibition of human MIAPaCa2 cells after 72 hrs by MTT assay
[PMID: 18640746]
NCI-H460 IC50
> 100 μM
Compound: 21
Growth inhibition of human H460 cells after 72 hrs by MTT assay
Growth inhibition of human H460 cells after 72 hrs by MTT assay
[PMID: 18640746]
SW-620 IC50
> 100 μM
Compound: 21
Growth inhibition of human SW620 cells after 72 hrs by MTT assay
Growth inhibition of human SW620 cells after 72 hrs by MTT assay
[PMID: 18640746]
Molecular Weight

125.13

Formula

C5H7N3O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC(C=CN1C)=NC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (79.92 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.9917 mL 39.9584 mL 79.9169 mL
5 mM 1.5983 mL 7.9917 mL 15.9834 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (392 KB)

COA (248 KB) HNMR (117 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.9917 mL 39.9584 mL 79.9169 mL 199.7922 mL
5 mM 1.5983 mL 7.9917 mL 15.9834 mL 39.9584 mL
10 mM 0.7992 mL 3.9958 mL 7.9917 mL 19.9792 mL
15 mM 0.5328 mL 2.6639 mL 5.3278 mL 13.3195 mL
20 mM 0.3996 mL 1.9979 mL 3.9958 mL 9.9896 mL
25 mM 0.3197 mL 1.5983 mL 3.1967 mL 7.9917 mL
30 mM 0.2664 mL 1.3319 mL 2.6639 mL 6.6597 mL
40 mM 0.1998 mL 0.9990 mL 1.9979 mL 4.9948 mL
50 mM 0.1598 mL 0.7992 mL 1.5983 mL 3.9958 mL
60 mM 0.1332 mL 0.6660 mL 1.3319 mL 3.3299 mL
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1-Methylcytosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1-Methylcytosine1122-47-0Nucleoside Antimetabolite/AnalogInhibitorinhibitorinhibit

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