1-Hydroxyphenazine (Synonyms: Hemipyocyanine; 1-Phenazinol; Hemi-pyocyanin)

Cat. No.: HY-W068682 Purity: 99.42%: FAQs
Data Sheet SDS COA Handling Instructions

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1-Hydroxyphenazine (Hemipyocyanine; 1-Phenazinol; Hemi-pyocyanin) is an inhibitor for α-Amylase with an IC₅₀ of 3.1 μg/mL. 1-Hydroxyphenazine exhibits anticancer and anti-inflammatory activity against cells A549, 1321N1 and RAW264.7, antifungal and antibacterial activity against strains Candida albicans, Aspergillus fumigatus, Escherichia coli and Xanthomonas campestris.

For research use only. We do not sell to patients.

1-Hydroxyphenazine Chemical Structure

CAS No. : 528-71-2

Size	Stock	Quantity
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Biological Activity
Purity & Documentation
References
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Description

1-Hydroxyphenazine (Hemipyocyanine; 1-Phenazinol; Hemi-pyocyanin) is an inhibitor for α-Amylase with an IC₅₀ of 3.1 μg/mL^[1]. 1-Hydroxyphenazine exhibits anticancer and anti-inflammatory activity against cells A549, 1321N1 and RAW264.7, antifungal and antibacterial activity against strains Candida albicans, Aspergillus fumigatus, Escherichia coli and Xanthomonas campestris^[2]^[3]^[4]^[5].

In Vitro

1-Hydroxyphenazine (0.1-100 μM) inhibits proliferation against brain astrocytoma 1321N1 cells through formation of acidic vesicular organelle, which is a hallmark of cell autophagy, exhibits potential toxicity in central nervous system (CNS)^[2].
Hydroxyphenazine (0-10 μg/mL) inhibits secretion of TNF-α and M1 cell polarization, inhibits LPS induced inflammation in RAW264.7 cells without significant toxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:	RAW264.7
Concentration:	0-10 μg/mL
Incubation Time:	24 h
Result:	Inhibited proliferation of RAW264.7 in a dose-dependent maner.

RT-PCR^[3]

Cell Line:	RAW264.7
Concentration:	0-10 μg/mL
Incubation Time:	24 h
Result:	Decreased levels of TNF-α, IL-1β and IL-6 mRNA expressions.

Molecular Weight

196.20

Formula

C₁₂H₈N₂O

CAS No.

528-71-2

Appearance

Solid

Color

Yellow to orange

SMILES

OC1=CC=CC2=NC3=CC=CC=C3N=C21

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (509.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0968 mL	25.4842 mL	50.9684 mL
5 mM	1.0194 mL	5.0968 mL	10.1937 mL
10 mM	0.5097 mL	2.5484 mL	5.0968 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (252 KB) HNMR (250 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Nguyen TH, et al., Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards. Mar Drugs. 2022 Apr 23;20(5):283. [Content Brief]

[2]. McFarland AJ, et al., Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity. Arch Toxicol. 2012 Feb;86(2):275-84 [Content Brief]

[3]. Xiao J, et al., Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells. Curr Microbiol. 2021 Jul;78(7):2762-2773. [Content Brief]

[4]. Prabhu MS, et al., Purification and molecular and biological characterisation of the 1-hydroxyphenazine, produced by an environmental strain of Pseudomonas aeruginosa. World J Microbiol Biotechnol. 2014 Dec;30(12):3091-9. [Content Brief]

[5]. Kerr JR, et al., Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth. J Clin Pathol. 1999 May;52(5):385-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.0968 mL	25.4842 mL	50.9684 mL	127.4210 mL
	5 mM	1.0194 mL	5.0968 mL	10.1937 mL	25.4842 mL
	10 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	15 mM	0.3398 mL	1.6989 mL	3.3979 mL	8.4947 mL
	20 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	25 mM	0.2039 mL	1.0194 mL	2.0387 mL	5.0968 mL
	30 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	40 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	50 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	60 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	80 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	100 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL

1-Hydroxyphenazine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1-Hydroxyphenazine528-71-2Hemipyocyanine 1-Phenazinol Hemi-pyocyaninAmylasesBacterialType 2 diabetehemi-pyocyaninA5491321N1RAW264.7Candida albicansAspergillus fumigatusEscherichia coliXanthomonas campestrisInhibitorinhibitorinhibit

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