2',4'-Dihydroxylchalcone (Synonyms: 2',4'-DHC)

Cat. No.: HY-W109754 Purity: 98.78%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

2',4'-Dihydroxychalcone, in combination with nalidixic acid (HY-B0398), exhibits synergistic effects against E. coli by reducing membrane permeability.

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2',4'-Dihydroxylchalcone Chemical Structure

CAS No. : 1776-30-3

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
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Description

2',4'-Dihydroxychalcone, in combination with nalidixic acid (HY-B0398), exhibits synergistic effects against E. coli by reducing membrane permeability^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 19	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
A549	IC₅₀	23.2 μM Compound: 8	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 19689125]
A549	IC₅₀	41.6 μM Compound: 27	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 18440233]
B16-BL6	IC₅₀	> 100 μM Compound: 8	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 19689125]
B16-BL6	IC₅₀	44.3 μM Compound: 27	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 18440233]
BJ	IC₅₀	262.5 μM Compound: 3a	Cytotoxicity against human BJ cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human BJ cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
C2C12	IC₅₀	32.26 μg/mL Compound: 12	Cytotoxicity against mouse C2C12 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse C2C12 cells assessed as reduction in cell viability by MTT assay	[PMID: 33412152]
HEK293	IC₅₀	109 μM Compound: 6	Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay	[PMID: 26473275]
HeLa	IC₅₀	23.1 μM Compound: 27	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 18440233]
HeLa	IC₅₀	24.4 μM Compound: 8	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 19689125]
HL-60	IC₅₀	6.3 μM Compound: 3	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 25091929]
HT-1080	IC₅₀	17 μM Compound: 8	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 19689125]
HT-1080	IC₅₀	34 μM Compound: 27	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 18440233]
Lewis lung carcinoma cell line	IC₅₀	39.5 μM Compound: 27	Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay	[PMID: 18440233]
LoVo	IC₅₀	81 μM Compound: 19	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
MCF7	IC₅₀	> 10 μM Compound: 2d	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
MCF7	IC₅₀	> 50 μM Compound: 3a	Antiproliferative activity against human MCF7 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Antiproliferative activity against human MCF7 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
MDA-MB-231	IC₅₀	> 10 μM Compound: 2d	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
NALM-6	IC₅₀	18 μM Compound: 3	Cytotoxicity against human NALM6 cells by MTT assay Cytotoxicity against human NALM6 cells by MTT assay	[PMID: 25091929]
PC-3	IC₅₀	> 100 μM Compound: 19	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
PC-3	IC₅₀	13.75 μM Compound: 3a	Antiproliferative activity against human PC3 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay Antiproliferative activity against human PC3 cells after 48 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 30482548]
Peritoneal macrophage cell	CC₅₀	416.7 μM Compound: DHC	Cytotoxicity against Swiss albino mouse peritoneal macrophages after 24 hrs by MTT assay Cytotoxicity against Swiss albino mouse peritoneal macrophages after 24 hrs by MTT assay	[PMID: 26169126]
SK-MEL-28	IC₅₀	75 μM Compound: 19	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-373MG ATCC	IC₅₀	> 100 μM Compound: 19	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
WM-115	IC₅₀	42 μM Compound: 3	Cytotoxicity against human WM115 cells by MTT assay Cytotoxicity against human WM115 cells by MTT assay	[PMID: 25091929]

Molecular Weight

240.25

Formula

C₁₅H₁₂O₃

CAS No.

1776-30-3

Appearance

Solid

Color

Yellow to orange

SMILES

O=C(C1=CC=C(O)C=C1O)/C=C/C2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (416.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1623 mL	20.8117 mL	41.6233 mL
5 mM	0.8325 mL	4.1623 mL	8.3247 mL
10 mM	0.4162 mL	2.0812 mL	4.1623 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (267 KB) SDS (393 KB)

COA (246 KB) HNMR (230 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Septama A W, et al. Prospect of plant-based flavonoids to overcome antibacterial resistance: A mini-review. Walailak Journal of Science and Technology (WJST), 2020, 17(5): 503-513.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1623 mL	20.8117 mL	41.6233 mL	104.0583 mL
	5 mM	0.8325 mL	4.1623 mL	8.3247 mL	20.8117 mL
	10 mM	0.4162 mL	2.0812 mL	4.1623 mL	10.4058 mL
	15 mM	0.2775 mL	1.3874 mL	2.7749 mL	6.9372 mL
	20 mM	0.2081 mL	1.0406 mL	2.0812 mL	5.2029 mL
	25 mM	0.1665 mL	0.8325 mL	1.6649 mL	4.1623 mL
	30 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	40 mM	0.1041 mL	0.5203 mL	1.0406 mL	2.6015 mL
	50 mM	0.0832 mL	0.4162 mL	0.8325 mL	2.0812 mL
	60 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	80 mM	0.0520 mL	0.2601 mL	0.5203 mL	1.3007 mL
	100 mM	0.0416 mL	0.2081 mL	0.4162 mL	1.0406 mL

2',4'-Dihydroxylchalcone Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2',4'-Dihydroxylchalcone1776-30-32',4'-DHCBacterialnalidixic acidE. colimembrane permeabilityInhibitorinhibitorinhibit

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2',4'-Dihydroxylchalcone (Synonyms: 2',4'-DHC)

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2',4'-Dihydroxylchalcone Related Antibodies

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Complete Stock Solution Preparation Table

2',4'-Dihydroxylchalcone Related Classifications

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